Mouse IFN-alpha ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IFN-alpha ELISA Kit Summary

• Specificity: Mouse IFN-alpha
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IFN-alpha ELISA Kit

• IFNA
• IFNA2
• IFNA2b
• IFNalpha
• IFN-alpha
• IFN-alphaA
• INFA2
• interferon alpha 2
• LeIF D

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Publications for IFN-alpha (42120-1)(27)

Publications using 42120-1

• Krzyzowska, M;Janicka, M;Chodkowski, M;Patrycy, M;Obuch-Woszczaty?ska, O;Tomaszewska, E;Ranoszek-Soliwoda, K;Celichowski, G;Grobelny, J; Epigallocatechin Gallate-Modified Silver Nanoparticles Show Antiviral Activity against Herpes Simplex Type 1 and 2 Viruses 2023-09-29 [PMID: 37896801] (Mouse)
• Y Song, F Wei, Y Liu, F Han, L Ma, Y Zhuang, C Pan, Z Jia, A Gong IL-33/ST2 Activation Is involved in Ro60-Regulated Photosensitivity in Cutaneous Lupus Erythematosus Oncogene, 2022-07-20;2022(0):4955761. 2022-07-20 [PMID: 35909659] (Mouse)
• G Wan, Y An, J Tao, Y Wang, Q Zhou, R Yang, Q Liang MicroRNA-129-5p alleviates spinal cord injury in mice via suppressing the apoptosis and inflammatory response through HMGB1/TLR4/NF-&kappaB pathway Biosci. Rep., 2020-03-27;40(3):. 2020-03-27 [PMID: 32096822] (Mouse)
• S Guo, M Vieweger, K Zhang, H Yin, H Wang, X Li, S Li, S Hu, A Sparreboom, BM Evers, Y Dong, W Chiu, P Guo Ultra-thermostable RNA nanoparticles for solubilizing and high-yield loading of paclitaxel for breast cancer therapy Nat Commun, 2020-02-20;11(1):972. 2020-02-20 [PMID: 32080195] (Mouse)
• O Danov, L Lasswitz, H Obernolte, C Hesse, A Braun, S Wronski, K Sewald Rupintrivir reduces RV-induced TH-2 cytokine IL-4 in precision-cut lung slices (PCLS) of HDM-sensitized mice ex vivo Respir. Res., 2019-10-22;20(1):228. 2019-10-22 [PMID: 31640701] (Mouse)
• K Wang, H Ma, H Liu, W Ye, Z Li, L Cheng, L Zhang, Y Lei, L Shen, F Zhang The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses Cell Rep, 2019-05-14;27(7):2075-2091.e5. 2019-05-14 [PMID: 31091447] (Mouse)
• BG Lania, J Morari, AR Almeida, MND Silva, G Vieira-Dam, KA Lins, CL César, LA Velloso, NB Maia, ML Cintra, PENF Velho Topical essential fatty acid oil on wounds: Local and systemic effects PLoS ONE, 2019-01-04;14(1):e0210059. 2019-01-04 [PMID: 30608959] (Mouse)
• S Fujiwara, M Hoshizaki, Y Ichida, D Lex, E Kuroda, KJ Ishii, S Magi, M Okada, H Takao, M Gandou, H Imai, R Hara, H Herzog, A Yoshimura, H Okamura, JM Penninger, AS Slutsky, S Uhlig, K Kuba, Y Imai Pulmonary phagocyte-derived NPY controls the pathology of severe influenza virus infection Nat Microbiol, 2018-11-19;4(2):258-268. 2018-11-19 [PMID: 30455472] (Mouse)
• YW Lin, LC Wang, CK Lee, SH Chen Flt3 ligand treatment reduces enterovirus A71 lethality in mice with enhanced B cell responses Sci Rep, 2018-08-15;8(1):12184. 2018-08-15 [PMID: 30111869] (Mouse)
• S Kimura, T Matsumiya, Y Shiba, M Nakanishi, R Hayakari, S Kawaguchi, H Yoshida, T Imaizumi The Essential Role of Double-Stranded RNA-Dependent Antiviral Signaling in the Degradation of Nonself Single-Stranded RNA in Nonimmune Cells J. Immunol., 2018-06-20;0(0):. 2018-06-20 [PMID: 29925678] (Mouse)
• Sujin An, Yung Jin Jeon, Ara Jo, Hyun Jung Lim, Young Eun Han, Sung Woo Cho et al. Initial Influenza Virus Replication Can Be Limited in Allergic Asthma Through Rapid Induction of Type III Interferons in Respiratory Epithelium Frontiers in Immunology 2018-05-17 [PMID: 29867963] (Mouse)
• P Xia, S Wang, B Ye, Y Du, C Li, Z Xiong, Y Qu, Z Fan A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion Immunity, 2018-04-03;0(0):. 2018-04-03 [PMID: 29625897] (Mouse)
• Joerg Mattes TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection Am. J. Physiol. Lung Cell Mol. Physiol., 2016-11-11;0(0):ajplung.00200. 2016-11-11 [PMID: 27836899] (Mouse)
• Zhao D, Xu A, Dai Z, Peng J, Zhu M, Shen J, Zheng Q, Ran Z WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features. BMC Gastroenterol, 2015-12-10;15(0):173. 2015-12-10 [PMID: 26652024] (Mouse)
• Chan , Mei Po, Onji , Masahiro, Fukui , Ryutaro, Kawane , Kohki, Shibata , Takuma, Saitoh , Shin-ich, Ohto , Umeharu, Shimizu , Toshiyuk, Barber , Glen N, Miyake , Kensuke DNase II-dependent DNA digestion is required for DNA sensing by TLR9. Nat Commun, 2015-01-20;6(0):5853. 2015-01-20 [PMID: 25600358] (Mouse)
• Desch , A Nicole, Gibbings , Sophie L, Clambey , Eric T, Janssen , William, Slansky , Jill E, Kedl , Ross M, Henson , Peter M, Jakubzick , Claudia Dendritic cell subsets require cis-activation for cytotoxic CD8 T-cell induction. Nat Commun, 2014-08-19;5(0):4674. 2014-08-19 [PMID: 25135627] (Mouse)
• Aeffner F, Woods P, Davis I Activation of A1-adenosine receptors promotes leukocyte recruitment to the lung and attenuates acute lung injury in mice infected with influenza A/WSN/33 (H1N1) virus. J Virol, 2014-06-25;88(17):10214-27. 2014-06-25 [PMID: 24965449] (Mouse)
• Dulek D, Newcomb D, Toki S, Goliniewska K, Cephus J, Reiss S, Bates J, Crowe J, Boyd K, Moore M, Zhou W, Peebles R STAT4 deficiency fails to induce lung Th2 or Th17 immunity following primary or secondary respiratory syncytial virus (RSV) challenge but enhances the lung RSV-specific CD8+ T cell immune response to secondary challenge. J Virol, 2014-06-11;88(17):9655-72. 2014-06-11 [PMID: 24920804] (Mouse)
• Bauer E, Zheng H, Lotze M, Bauer P Recombinant human interferon alpha 2b prevents and reverses experimental pulmonary hypertension. PLoS ONE, 2014-05-16;9(5):e96720. 2014-05-16 [PMID: 24837600] (Mouse)
• Jayaraman A, Jackson D, Message S, Pearson R, Aniscenko J, Caramori G, Mallia P, Papi A, Shamji B, Edwards M, Westwick J, Hansel T, Stanciu L, Johnston S, Bartlett N IL-15 complexes induce NK- and T-cell responses independent of type I IFN signaling during rhinovirus infection. Mucosal Immunol, 2014-01-29;7(5):1151-64. 2014-01-29 [PMID: 24472849] (Mouse)
• Berri F, Rimmelzwaan G, Hanss M, Albina E, Foucault-Grunenwald M, Le V, Vogelzang-van Trierum S, Gil P, Camerer E, Martinez D, Lina B, Lijnen R, Carmeliet P, Riteau B Plasminogen controls inflammation and pathogenesis of influenza virus infections via fibrinolysis. PLoS Pathog, 2013-03-21;9(3):e1003229. 2013-03-21 [PMID: 23555246] (Mouse)
• Sun Y, Liu G, Li Z, Chen Y, Liu Y, Liu B, Su Z Modulation of dendritic cell function and immune response by cysteine protease inhibitor from murine nematode parasite Heligmosomoides polygyrus. Immunology, 2013-04-01;138(4):370-81. 2013-04-01 [PMID: 23240853] (Mouse)
• Bondue B, Vosters O, de Nadai P, Glineur S, De Henau O, Luangsay S, Van Gool F, Communi D, De Vuyst P, Desmecht D, Parmentier M ChemR23 dampens lung inflammation and enhances anti-viral immunity in a mouse model of acute viral pneumonia. PLoS Pathog., 2011-11-03;7(11):e1002358. 2011-11-03 [PMID: 22072972] (Mouse)
• Walsh KB, Teijaro JR, Wilker PR Suppression of cytokine storm with a sphingosine analog provides protection against pathogenic influenza virus. Proc. Natl. Acad. Sci. U.S.A., 2011-06-29;108(29):12018-23. 2011-06-29 [PMID: 21715659] (Mouse)
• Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines. J. Immunol., 2011-03-09;186(8):4707-15. 2011-03-09 [PMID: 21389257] (Mouse)
• Guerra S, Gonzalez JM, Climent N, Reyburn H, Lopez-Fernandez LA, Najera JL, Gomez CE, Garcia F, Gatell JM, Gallart T, Esteban M Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS. J. Virol., 2010-06-09;84(16):8141-52. 2010-06-09 [PMID: 20534857] (Mouse)
• Qin L, Greenland JR, Moriya C Effects of type I interferons on the adjuvant properties of plasmid granulocyte-macrophage colony-stimulating factor in vivo. J. Virol., 2007-07-25;81(19):10606-13. 2007-07-25 [PMID: 17652387] (Mouse)

FAQs for IFN-alpha (42120-1). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.