IFN-alpha Antibodies

1082-CR) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Cotton Rat IFN-a (Cys109Tyr) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1082). The ND₅₀ is typically 0.015-0.065 µg/mL." class="big_lightbox"> 1082-CR) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Cotton Rat IFN-a (Cys109Tyr) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1082). The ND₅₀ is typically 0.015-0.065 µg/mL." alt="Recombinant Cotton Rat IFN-a (Cys109Tyr) (Catalog # 1082-CR) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Cotton Rat IFN-a (Cys109Tyr) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1082). The ND₅₀ is typically 0.015-0.065 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IFN-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs; left panel, positive stain) and MCF-7 human breast cancer cell line (right panel, negative stain) using Mouse Anti-Human IFN-a Monoclonal Antibody (Catalog # MAB93454) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />