MOCS2 Overexpression Lysate

Western Blot: MOCS2 Overexpression Lysate (Adult Normal) [NBL1-13175] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MOCS2.

• Reactivity: Hu
• Applications: WB

MOCS2 Overexpression Lysate Summary

• Description:

  MOCS2 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC208485
  
  Accession#: NM_004531
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: MOCS2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 20.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for MOCS2 Overexpression Lysate

• EC 2.-
• MCBPE
• MOCO1-A
• MOCO1-B
• MOCO1MOCS2B
• MOCS2A
• molybdenum cofactor biosynthesis protein E
• molybdenum cofactor synthesis 2
• Molybdenum cofactor synthesis protein 2 large subunit
• Molybdenum cofactor synthesis protein 2 small subunit
• Molybdenum cofactor synthesis protein 2A
• Molybdenum cofactor synthesis protein 2B
• molybdopterin synthase catalytic subunit
• molybdopterin synthase sulfur carrier subunit
• Molybdopterin-synthase large subunit
• Molybdopterin-synthase small subunit
• MPT synthase large subunit
• MPTS
• Sulfur carrier protein MOCS2A

Background

Eukaryotic molybdoenzymes use a unique molybdenum cofactor (MoCo) consisting of a pterin, termed molybdopterin, and the catalytically active metal molybdenum. MoCo is synthesized from precursor Z by the heterodimeric enzyme molybdopterin synthase. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. The proteins were initially thought to be encoded from a bicistronic transcript. They are now thought to be encoded from monocistronic transcripts. Alternatively spliced transcripts have been found for this locus that encode the large and small subunits. [provided by RefSeq]. Transcript Variant: This variant (3) encodes the large subunit MOCS2B, also called MOCO1-B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MOCS2