Mouse MMP-9 ELISA Kit (Colorimetric) Summary
Standard Curve Range |
0.625 ng/ml-40 ng/ml |
Sensitivity |
0.552 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 10 |
Intra-Assay |
%CV < 8 |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
MMP9 |
Applications/Dilutions
Packaging, Storage & Formulations
Notes
Please contact our Technical Services with any questions regarding species reactivity
Alternate Names for Mouse MMP-9 ELISA Kit (Colorimetric)
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for MMP-9 ELISA Kit (NBP2-60095). (Showing 1 - 1 of 1 FAQ).
-
I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any?
- We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)
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