Mouse MMP-9 ELISA Kit (Colorimetric)

ELISA: Mouse MMP-9 ELISA Kit (Colorimetric) [NBP2-60095] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Mouse MMP-9 ELISA Kit (Colorimetric) [NBP2-60095] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 0.625 ng/mL - 40 ng/mL
• Sensitivity: 0.552 ng/mL

Mouse MMP-9 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Standard Curve Range: 0.625 ng/mL - 40 ng/mL
• Sensitivity: 0.552 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMP9

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse MMP-9 ELISA Kit (Colorimetric)

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for MMP-9 ELISA Kit (NBP2-60095)(1)

Publication using NBP2-60095

• Oue H, Yamazaki Y, Qiao W et al. LRP1 in vascular mural cells modulates cerebrovascular integrity and function in the presence of APOE4 JCI insight 2023-04-10 [PMID: 37036005] (ELISA, Mouse)

FAQs for MMP-9 ELISA Kit (NBP2-60095). (Showing 1 - 2 of 2 FAQ).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.
2. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: MMP9