MMP-25/MT6-MMP Antibody (141811) [Unconjugated]

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MMP-25/MT6-MMP Monoclonal Antibody (Catalog # MAB11422, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 141811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-25/MT6-MMP Antibody (141811) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP‑25/MT6-MMP
  Pro314-Cys508
  Accession # Q9NPA2
• Specificity: Detects human MMP-25/MT6-MMP in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP25
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-25/MT6-MMP Antibody (141811) [Unconjugated]

• Leukolysin
• matrix metallopeptidase 25
• matrix metallopeptidase-like 1
• matrix metalloproteinase 20
• matrix metalloproteinase 25
• matrix metalloproteinase-25
• matrix metalloproteinase-like 1
• membrane-type 6 matrix metalloproteinase
• membrane-type matrix metalloproteinase 6
• membrane-type-6 matrix metalloproteinase
• MMP20
• MMP20A
• MMP25
• MMP-25
• MMPL1
• MT6MMP
• MT6-MMP
• MT-MMP 6
• MTMMP6
• MT-MMP6

Background

MMP-25, also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. MMP-25 cleaves collagen type 4, gelatin, fibronectin, and fibrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MMP25
• Uniprot:
  • Q9NPA2()