MMP-19 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

MMP-19 Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP-19
  Leu229-Tyr508
  Accession # Q99542
• Specificity: Detects human MMP-19 in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-19 Antibody [Alexa Fluor® 647]

• EC 3.4.24.-
• matrix metallopeptidase 19
• matrix metalloproteinase 18
• matrix metalloproteinase 19
• Matrix metalloproteinase RASI
• Matrix metalloproteinase-18
• matrix metalloproteinase-19
• MMP-18
• MMP18MMP-19
• MMP19
• MMP-19
• RASI
• RASI-1

Background

MMP-19 (Matrix metalloprotease 19; also MMP-18 and MMP RASI) is a 55-59 kDa member of the peptidase M10A family of enzymes. It is widely expressed, being secreted by stratum basale keratinocytes, smooth muscle cells, epiphysial cartilage chondrocytes and monocytes/macrophages. MMP-19 has multiple substrates, including components of the basement membrane (type IV collagen; laminin; nidogen), fibronectin, aggrecan plus COMP, and IGFBP3, this latter cleavage resulting in the release of active IGF-I. Studies involving MMP-19 demonstrate an antiangiogenic function. This is attributable to the processing of plasminogen, generating angiostatin-like molecules, and the creation of an environment that promotes the ECM retention of soluble VEGF. Human proMMP-19 is 490 amino acids (aa) in length. It contains an autocleavable 9 kDa propeptide (aa 19-97) plus a 411 aa mature region (aa 98-508). The mature region contains a Zn catalytic region (aa 103-256) plus four hemopexin-like domains (aa 293-508). There are three additional potential isoforms. One shows an 88 aa substitution for aa 300-508, a second contains a 12 aa substitution for aa 1-298, while a third possesses an alternative start site at Met80. Over aa 229-508, human MMP-19 shares 76% aa identity with mouse MMP-19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.