MMP-13 Antibody

Images

 
Western Blot: MMP-13 Antibody [NBP1-45723] - Analysis of MMP-13 expression with Jurkat cell lysate.
Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody [NBP1-45723] - MMP-13 antibody was tested at 1:50 in MDA-MB-231 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Western Blot: MMP-13 Antibody [NBP1-45723] - Analysis of MMP-13 in human full-length recombinant protein using anti-MMP-13 antibody. Image from verified customer review.
Simple Western: MMP-13 Antibody [NBP1-45723] - Simple Western lane view shows a specific band for MMP-13 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Eq, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MMP-13 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 454 of rat MMP-13.
Specificity
The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMP13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunoprecipitation
Reviewed Applications
Read 1 Review rated 3
using
NBP1-45723 in the following applications:

Publications
Read Publication using
NBP1-45723 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MMP-13 Antibody

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

Collagenase-3 (MMP13), a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, is expressed in the skeleton during embryonic development and is highly overexpressed in human carcinomas and in chondrocytes and synovial cells in rheumatoid arthritis and osteoarthritis. Findings demonstrate a unique role of Mmp13 in skeletal development (1). In arthritis, the destruction of fibrillar (type II) collagen is one of the hallmarks, with MMP-1 and MMP-13 being identified as key players in arthritic cartilage. MMP-13, furthermore, has been found in highly metastatic tumors. In contrast to many human MMPs, the MMP-13 distribution pattern is restrictive in normal tissues and selective in pathological conditions. MMP-13 demonstrates versatility in its substrate utilization. In addition to being highly active on type II collagen, MMP-13 cleaves other substrates, mostly macromolecules of the extracellular matrix, but also molecules such as connective tissue (CTGF) and fibrinogen. MMP-13 is controlled at multiple levels: i.e., the expression/synthesis, activation, and inhibition of the active enzyme (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Eq, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IP
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MMP-13 Antibody (NBP1-45723)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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(1)
WB
(1)
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Review for MMP-13 Antibody (NBP1-45723) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-45723:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot MMP-13 NBP1-45723
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reviewed by:
Anonymous
WB Human 02/03/2015
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Summary

ApplicationWestern Blot
Sample TestedRecombinant protein
SpeciesHuman

Blocking

Blocking Details5% milk in TBST 1 hr

Primary Anitbody

Dilution Ratio1ug/ml MMP13 antibody, overnight, 4 degrees, in 5% milk

Secondary Antibody

Secondary Descriptiongoat anti-rabbit
Secondary Concentration1:20,000

Details

Detection NotesECL, Western lightning (Perkin-Elmer), 1 min.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MMP-13 Antibody (NBP1-45723) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for MMP-13 Antibody (NBP1-45723)

Discover related pathways, diseases and genes to MMP-13 Antibody (NBP1-45723). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MMP-13 Antibody (NBP1-45723)

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Pathways for MMP-13 Antibody (NBP1-45723)

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PTMs for MMP-13 Antibody (NBP1-45723)

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Research Areas for MMP-13 Antibody (NBP1-45723)

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Blogs on MMP-13

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Calculators

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Recent Reviews

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Anonymous
02/03/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol MMP13
Entrez
Uniprot