MMP-13 Antibody (VIIIA2)

Immunohistochemistry-Paraffin: MMP-13 Antibody (VIIIA2) [NB110-5919] - Human placenta stained with MMP-13 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, IHC, IP
• Clone: VIIIA2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.4 mg/ml

MMP-13 Antibody (VIIIA2) Summary

• Description: Novus Biologicals Mouse MMP-13 Antibody (VIIIA2) (NB110-5919) is a monoclonal antibody validated for use in IHC, WB and IP. Anti-MMP-13 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant collagenase-3 protein
• Localization: Secreted
• Specificity: Recognizes pro and acive forms of MMP 13.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP13
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:12.5 - 1:25
  • Immunoprecipitation 1:25 - 1:50
  • Western Blot 2 - 4 ug/ml

Reactivity Notes

Rat reactivity reported in scientific literature (see Acta Odontol Scand. 2008). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.4 mg/ml
• Purity: Protein G purified

Alternate Names for MMP-13 Antibody (VIIIA2)

• CLG 3
• CLG3EC 3.4.24
• collagenase 3
• EC 3.4.24.-
• EC 3.4.24.22
• EC 3.4.24.24
• EC 3.4.24.35
• EC 3.4.24.65
• EC 3.4.24.7
• MANDP1
• matrix metallopeptidase 13 (collagenase 3)
• matrix metalloproteinase 13 (collagenase 3)
• Matrix metalloproteinase-13
• MMP13
• MMP-13

Background

Collagenase 3 (MMP13) is a secreted 452 residue protein which is released from cells as aninactive zymogen and activated extracellularly by removal of its propeptide. MMP13 was first identified in tumor cells but has since been found in synoviocytes and normal fibroblasts stimulated by IL6 or a combination of TNF alpha and IL1. Under normal conditions, MMP13 is expressed during embryogenesis (fetal bone development) and is present only at very low levels in adult tissue. However, this enzyme is reported to be involved in the development and metastasis of breast and lung carcinomas, chondrosarcomas and osteosarcomas, head and neck carcinomas and some forms of skin cancer. Additionally, this enzyme plays an important role in degenerative bone diseases including osteoarthritis and rheumatoid arthritis. In rodents, MMP13 is thought to fill the role of MMP1, which they appear to lack.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-13 Antibody (NB110-5919)(4)

Publications using NB110-5919

• Paraliov A, Vicaş R, Dîrnu R et al. Hip osteoarthritis – histopathological aspects Romanian Journal of Morphology and Embryology 2025-01-01 [PMID: 40384207]
• Liu J, Xu Y, Huang Y et al. Collagen membrane loaded with doxycycline through hydroxypropyl chitosan microspheres for the early reconstruction of alkali-burned cornea International journal of biological macromolecules 2023-06-01 [PMID: 37270120] (IHC)
• Katta M, Cump?t? CN, ?uculin? MJ et al. Clinical, histopathological and immunohistochemical changes of the superficial marginal periodontium caused by orthodontic treatment with fixed metallic orthodontic appliances Romanian Journal of Morphology and Embryology 2022-11-15 [PMID: 36374148] (Immunohistochemistry, Human)
• Berceanu C, Mehedintu C, Berceanu S et al. Morphological and ultrasound findings in multiple pregnancy placentation. Rom J Morphol Embryol 2018-09-03 [PMID: 30173248] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: MMP13
• Entrez:
  • 4322(Human)
  • 17386(Mouse)
  • 100008685(Rabbit)
• Uniprot:
  • P45452()