Porcine MMP-10 ELISA Kit (Chemiluminescence)

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma and other biological fluids
Standard Curve Range
0.55 - 400 pg/mL (example only; lot dependent)
Sensitivity
0.22 pg/mL (example only; lot dependent)

Order Details

Porcine MMP-10 ELISA Kit (Chemiluminescence) Summary

Description
Assay time: 2 hours, 40 minutes
Standard Curve Range
0.55 - 400 pg/mL (example only; lot dependent)
Sensitivity
0.22 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Spike Recovery
82 - 93% (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
MMP10

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Substrate A
  11. Substrate B
  12. Wash Buffer (30 x concentrate)

Alternate Names for Porcine MMP-10 ELISA Kit (Chemiluminescence)

  • EC 3.4.24
  • EC 3.4.24.22
  • matrix metallopeptidase 10 (stromelysin 2)
  • matrix metalloprotease 10
  • matrix metalloproteinase 10 (stromelysin 2)
  • Matrix metalloproteinase-10
  • MMP10
  • MMP-10
  • SL-2
  • STMY2stromelysin-2
  • Stromelysin 2
  • transin 2
  • transin-2

Background

Matrix metalloproteinases belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM), thus they play an important role in apoptosis, and tumor cell growth, invasion, metastasis, as well as in angiogenesis and wound healing. Most of the MMP's contain a common domain structures, which include a signal sequence, a propeptide, a catalytic domain and a hemopexin-like (Hpx) domain. MMP10 belongs to the Stromelysin group of proteases. Stromelysins degrade ECM proteoglycans, laminin, fibronectin and gelatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MMP-10 ELISA Kit (NBP3-42659). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol MMP10