Human MMP-10 ELISA Kit (Colorimetric) Summary
| Sensitivity |
3 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
MMP10 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
MMP10 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP10. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent (Item E): 5x concentrated buffered. For Standard/Sample(serum/plasma samples/cell culture medium/urine) diluent.
- Detection Antibody MMP-10 (Item F): biotinylated anti-human MMP-10(each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 600x concentratedHRP-conjugated streptavidin.
- MMP-10 Microplate (Item A): 96 wells (12 strips x 8 wells) coated withanti-human MMP-10.
- Standards (Item C), recombinant human MMP-10.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- Wash Buffer Concentrate (20x) (Item B)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human MMP-10 ELISA Kit (Colorimetric)
Background
Matrix metalloproteinases belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM), thus they play an important role in apoptosis, and tumor cell growth, invasion, metastasis, as well as in angiogenesis and wound healing. Most of the MMP's contain a common domain structures, which include a signal sequence, a propeptide, a catalytic domain and a hemopexin-like (Hpx) domain. MMP10 belongs to the Stromelysin group of proteases. Stromelysins degrade ECM proteoglycans, laminin, fibronectin and gelatin.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for MMP-10 ELISA Kit (KA1750). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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