MMP-10 Antibody (117239) [Unconjugated]

Co-localisation of MMPs with markers of classical activation in human atherosclerotic plaques.(A) Serial sections were stained by peroxidase with anti-CD68 (A. CD68) and by dual immuno-fluorescence with anti-MMP-1 or anti-MMP-10 (red) together with anti-COX-2 or p65RelA (green) as shown, and the images were superimposed digitally. Nuclear localised p65RelA (NF-kB*) was detected because of the shift in nuclear counterstain colour from dark blue (DAPI alone) to sky blue (blue plus green) in the superimposed image. (B) Areas rich in macrophages identified from the peroxidase stain were identified in the serial section. Cells in the whole field were counted and a percentage of each staining pattern calculated. Values are means ± SEM, n = 6, * p<0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22880008), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, IP, B/N
• Clone: 117239
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MMP-10 Antibody (117239) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP-10
  Tyr18-Cys476
  Accession # P09238
• Specificity: Detects the pro and active forms of human MMP-10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -12, or -13 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 1 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-10 Antibody (117239) [Unconjugated]

• EC 3.4.24
• EC 3.4.24.22
• matrix metallopeptidase 10 (stromelysin 2)
• matrix metalloprotease 10
• matrix metalloproteinase 10 (stromelysin 2)
• Matrix metalloproteinase-10
• MMP10
• MMP-10
• SL-2
• STMY2stromelysin-2
• Stromelysin 2
• transin 2
• transin-2

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-10 Antibody (MAB9101)(6)

Publications using MAB9101

• PL Chi, CC Cheng, CC Hung, MT Wang, HY Liu, MW Ke, MC Shen, KC Lin, SH Kuo, PP Hsieh, SR Wann, WC Huang MMP-10 from M1 macrophages promotes pulmonary vascular remodeling and pulmonary arterial hypertension International journal of biological sciences, 2022-01-01;18(1):331-348. 2022-01-01 [PMID: 34975336] (IHC, Human)
• Juan Marta-Enguita, Manuel Navarro-Oviedo, Roberto Muñoz, Jorge Olier-Arenas, Guillermo Zalba, Ramon Lecumberri et al. Inside the Thrombus: Association of Hemostatic Parameters With Outcomes in Large Vessel Stroke Patients Frontiers in Neurology 2/22/2021 [PMID: 33692737]
• R Reyes, JA Rodríguez, J Orbe, MR Arnau, C Évora, A Delgado Combined sustained release of BMP2 and MMP10 accelerates bone formation and mineralization of calvaria critical size defect in mice Drug Deliv, 2018-11-01;25(1):750-756. 2018-11-01 [PMID: 29516759] (Western Blot, Human)
• Ye Y, Pringle LM, Lau AW TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB. Oncogene, 2010-04-26;29(25):3619-29. 2010-04-26 [PMID: 20418905] (Western Blot, Human)
• Saghizadeh M, Kramerov AA, Yaghoobzadeh Y et al. Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes. Brain Res Bull 2/1/2010 [PMID: 19828126]
• Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3. J. Cell Biol., 2006-10-09;175(1):179-91. 2006-10-09 [PMID: 17030988] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MMP10
• Uniprot:
  • P09238()