MMACHC Antibody - BSA Free

Western Blot: MMACHC Antibody [NBP1-32513] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with MMACHC antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: MMACHC Antibody [NBP1-32513] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: MMACHC Antibody [NBP1-32513] - C2C12 xenograft, using MMACHC antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: MMACHC Antibody [NBP1-32513] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MMACHC antibody [N1C2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: MMACHC Antibody [NBP1-32513] - Human hepatoma, using MMACHC antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MMACHC Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MMACHC. The exact sequence is proprietary.
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMACHC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MMACHC Antibody - BSA Free

• cblC
• DKFZP564I122
• FLJ25671
• methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria
• methylmalonic aciduria and homocystinuria type C protein
• RP11-291L19.3

Background

The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MMACHC
• Entrez:
  • 25974(Human)
  • 67096(Mouse)
• Uniprot:
  • Q9Y4U1(Human)