Recombinant Human MMAB His Protein

SDS-Page: Recombinant Human MMAB Protein [NBP1-48323] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human MMAB His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 33-250 of Human MMAB
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQSRGPQGVE DGDRPQPSSK TPRIPKIYTK TGDKGFSSTF TGERRPKDDQ VFEAVGTTDE LSSAIGFALE LVTEKGHTFA EELQKIQCTL QDVGSALATP CSSAREAHLK YTTFKAGPIL ELEQWIDKYT SQLPPLTAFI LPSGGKISSA LHFCRAVCRR AERRVVPLVQ MGETDANVAK FLNRLSDYLF TLARYAAMKE GNQEKIYKKN DPSAESEGL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MMAB
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 26.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human MMAB His Protein

• aquocob(I)alamin vitamin B12s adenosyltransferase
• ATP:cob(I)alamin adenosyltransferase
• ATP:corrinoid adenosyltransferase
• ATR
• cblB
• c-diamide adenosyltransferase, mitochondrial
• cob
• Cob(I)alamin adenosyltransferase
• cob(I)yrinic acid a
• EC 2.5.1.17
• methylmalonic aciduria (cobalamin deficiency) cblB type
• methylmalonic aciduria (cobalamin deficiency) type B
• Methylmalonic aciduria type B protein
• MGC20496

Background

MMAB is a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12 containing coenzyme for methylmalonyl-CoA mutase(MCM). Impaired MMAB activity leads to the inherited disorder vitamin B12 dependent methylmalonic aciduria linked to the cblB complementation group. Recombinant human MMAB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MMAB