MLKL Antibody (954702) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: IHC
• Clone: 954702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

MLKL Antibody (954702) [Alexa Fluor® 405] Summary

• Immunogen: Phosphopeptide containing the human MLKL T357 site
  Accession # Q8NB16
• Specificity: Detects human MLKL when phosphorylated at T357.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MLKL Antibody (954702) [Alexa Fluor® 405]

• FLJ34389
• mixed lineage kinase domain-like protein
• mixed lineage kinase domain-like
• MLKL

Background

Mixed Lineage Kinase Domain-Like (MLKL) is a 471 amino acid member of the protein kinase superfamily, but lacks several amino acid residues necessary for kinase activity.  Phosphorylation of MLKL at T357 and S358  by receptor-interacting protein 3 (RIP3) causes trimerization and translocation of MLKL from the cytosol to the plasma membrane, where it mediates TNF-alpha induced necroptosis through membrane degradation and calcium influx.  Low expression of MLKL has been found to be correlated with poor prognosis in cervical squamous cell carcinoma, pancreatic cancer, and ovarian cancer.  Conversely, high expression of RIPK3 and MLKL in esophageal and colon cancer is associated with poor overall survival, possibly by promoting inflammatory cytokine production, sustaining tumor growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.