MLKL Antibody (954702) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: IHC
• Clone: 954702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

MLKL Antibody (954702) [Alexa Fluor® 488] Summary

• Immunogen: Phosphopeptide containing the human MLKL T357 site
  Accession # Q8NB16
• Specificity: Detects human MLKL when phosphorylated at T357.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MLKL Antibody (954702) [Alexa Fluor® 488]

• FLJ34389
• mixed lineage kinase domain-like protein
• mixed lineage kinase domain-like
• MLKL

Background

Mixed Lineage Kinase Domain-Like (MLKL) is a 471 amino acid member of the protein kinase superfamily, but lacks several amino acid residues necessary for kinase activity.  Phosphorylation of MLKL at T357 and S358  by receptor-interacting protein 3 (RIP3) causes trimerization and translocation of MLKL from the cytosol to the plasma membrane, where it mediates TNF-alpha induced necroptosis through membrane degradation and calcium influx.  Low expression of MLKL has been found to be correlated with poor prognosis in cervical squamous cell carcinoma, pancreatic cancer, and ovarian cancer.  Conversely, high expression of RIPK3 and MLKL in esophageal and colon cancer is associated with poor overall survival, possibly by promoting inflammatory cytokine production, sustaining tumor growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8NB16()