MLK3 Antibody

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Western Blot: MLK3 Antibody [NBP1-45711] - Analysis of MLK3 expression with HeLa cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Concentration
0.5 mg/ml

Order Details

MLK3 Antibody Summary

Immunogen
Synthetic peptide mapping to the C-terminus of human MLK3.
Specificity
It does not cross-react with MLK1, MLK2 and other mixed lineage kinases.
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K11
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS, 1.0% BSA and 50% Glycerol
Preservative
0.02% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 0.5-4 ug/ml
Application Notes
The antibody recognizes 92 kDa MLK3 in Western blots.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-45711 in the following applications:

  • WB
    1 publication

Alternate Names for MLK3 Antibody

  • EC 2.7.11
  • EC 2.7.11.25
  • MAP3K11
  • MEKK11
  • mitogen-activated protein kinase kinase kinase 11
  • Mixed lineage kinase 3
  • MLK3
  • MLK3MLK-3
  • PTK1
  • PTK1protein-tyrosine kinase PTK1
  • RHOE
  • SH3 domain-containing proline-rich kinase
  • SPRK
  • SPRKMGC17114
  • Src-homology 3 domain-containing proline-rich kinase

Background

Mixed lineage kinase-3 (MLK-3) is a mitogen-activated kinase kinase kinase that mediates stress-activating protein kinase (SAPK)/c-Jun NH2-terminal kinase activation. MLK-3 and other MLK family kinases are characterized by the presence of multiple protein-protein interaction domains including a tandem leucine/isoleucine zipper (LZs) motif. Leucine zippers are known to mediate protein dimerization raising the possibility that the tandem leucine/isoleucine zippers may function as a dimerization motif of MLK-3 (1). MLK-3 has several interesting structural features including an SH3 domain in the absence of an SH2 domain, a region containing two leucine zippers with an adjacent carboxy-terminal basic region, and a proline rich region. (2). HPK1 has been found to phosphorylate MLK-3 activation loop in vitro, and Ser281 was found to be the major phosphorylation site, indicating that HPK1 also activates MLK-3 via phosphorylation of the kinase activation loop (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MLK3 Antibody (NBP1-45711)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MAP3K11
Entrez
Uniprot

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