MLH3 Antibody

Product Discontinued

MLH3 Antibody Summary

• Immunogen: Peptide with sequence RQSLQQSMPPCEPP corresponding to C-Terminus according to NP_001035197.1, NP_055196.2.
• Specificity: This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MLH3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunofluorescence
  • Peptide ELISA Detection limit 1:2000
• Application Notes: WB: No signal obtained yet but low background observed in HeLa lysate at up to 2ug/ml.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for MLH3 Antibody

• DNA mismatch repair protein Mlh3
• HNPCC7
• MGC138372
• mutL (E. coli) homolog 3
• mutL homolog 3 (E. coli)
• MutL protein homolog 3

Background

This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MLH3 Antibody (NB100-1071)(1)

Publication using NB100-1071

• Lipkin SM, Wang V, Jacoby R et al. MLH3: a DNA mismatch repair gene associated with mammalian microsatellite instability. Nat Genet 2000-01-01 [PMID: 10615123]

Bioinformatics

• Gene Symbol: MLH3
• Entrez:
  • 27030(Human)
• Uniprot:
  • NP_055196.(Human)