MLH1 Antibody

Images

 
Western Blot: MLH1 Antibody [NB100-197] - Nuclear extract (50 ug for WB; 1 mg for IP) from HeLa cells. Antibody used at 1 ug/ml for WB and 5 ug/mg lysate for IP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

MLH1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 700 and the C-terminus (residue 756) of human mutL homolog 1 using the numbering given in entry NP_000240.1 (GeneID 4292).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MLH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-4 ul
  • Proximity Ligation Assay 1:1000-1:10000
Positive Control
MLH1 Lysate (NBL1-13134)
Publications
Read Publication using NB100-197.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MLH1 Antibody

  • COCA2FCC2
  • DNA mismatch repair protein Mlh1
  • hMLH1
  • HNPCC
  • HNPCC2MGC5172
  • mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2)
  • mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
  • MutL protein homolog 1

Background

MLH1 is a DNA mismatch repair gene, mutations in MLH1 are implicated in hereditary nonpolyposis colorectal cancer (HNPCC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MLH1 Antibody (NB100-197)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MLH1 Antibody (NB100-197) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MLH1 Products

Bioinformatics Tool for MLH1 Antibody (NB100-197)

Discover related pathways, diseases and genes to MLH1 Antibody (NB100-197). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for MLH1 Antibody (NB100-197)

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Blogs on MLH1.

NER, BER, HR and Other DNA Repair Pathways
A large number of antibody assays are devoted to the study of nuclear excision repair (NER) proteins. However, there are a number of other DNA repair pathways, many of which are instigated by NER and share the same proteins. DNA repair antibodies are...  Read full blog post.

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Bioinformatics

Gene Symbol MLH1
Entrez
Uniprot