| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | IHC |
| Clone | OTI1C1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 650 |
| Immunogen | Recombinant protein fragment corresponding to amino acids 299-527 of human MLH1 (NP_000240) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLH1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
![E-Cadherin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "E-Cadherin Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-72725AF350 | |
| Alexa Fluor 405 | NBP2-72725AF405 | |
| Alexa Fluor 488 | NBP2-72725AF488 | |
| Alexa Fluor 532 | NBP2-72725AF532 | |
| Alexa Fluor 594 | NBP2-72725AF594 | |
| Alexa Fluor 647 | NBP2-72725AF647 | |
| Alexa Fluor 700 | NBP2-72725AF700 | |
| Alexa Fluor 750 | NBP2-72725AF750 | |
| Biotin | NBP2-72725B | |
| DyLight 350 | NBP2-72725UV | |
| DyLight 405 | NBP2-72725V | |
| DyLight 488 | NBP2-72725G | |
| DyLight 550 | NBP2-72725R | |
| DyLight 594 | NBP2-72725DL594 | |
| DyLight 650 | NBP2-72725C | |
| DyLight 680 | NBP2-72725FR | |
| DyLight 755 | NBP2-72725IR | |
| FITC | NBP2-72725F | |
| HRP | NBP2-72725H | |
| Janelia Fluor 549 | NBP2-72725JF549 | |
| Janelia Fluor 646 | NBP2-72725JF646 |
Diseases for MLH1 Antibody (NBP2-72725C)Discover more about diseases related to MLH1 Antibody (NBP2-72725C).
| Pathways for MLH1 Antibody (NBP2-72725C)View related products by pathway.
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PTMs for MLH1 Antibody (NBP2-72725C)Learn more about PTMs related to MLH1 Antibody (NBP2-72725C).
| Research Areas for MLH1 Antibody (NBP2-72725C)Find related products by research area.
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NER, BER, HR and Other DNA Repair Pathways A large number of antibody assays are devoted to the study of nuclear excision repair (NER) proteins. However, there are a number of other DNA repair pathways, many of which are instigated by NER and share the same proteins. DNA repair antibodies are... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MLH1 |
![Western Blot: MSH2 Antibody [NB100-621] - Detection of Human and Mouse MSH2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/MSH2-Antibody-Western-Blot-NB100-621-img0008.jpg)
![Immunohistochemistry-Paraffin: MSH2 Antibody [NB100-621] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MSH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/images2/MSH2-Antibody-Immunohistochemistry-Paraffin-NB100-621-img0003.jpg)
![Western Blot: MSH2 Antibody [NB100-621] - Detection of human and mouse MSH2 by Western blot and immunoprecipitation using NB100-621. Detection: ECL with a 3 minute exposure.](http://images.novusbio.com/images2/MSH2-Antibody-Western-Blot-NB100-621-img0001.jpg)
![Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mouse mAb against HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate.](http://images.novusbio.com/images2/MSH6-Antibody-(3E1)-Western-Blot-NBP2-37537-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MSH6 Antibody (3E1) [NBP2-37537] - Immunofluorescence analysis of Hela cells using MSH6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/MSH6-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP2-37537-img0004.jpg)
![Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) [NBP2-37537] - Analysis of colon cancer tissues using MSH6 mouse mAb with DAB staining.](http://images.novusbio.com/images2/MSH6-Antibody-(3E1)-Immunohistochemistry-NBP2-37537-img0007.jpg)
![Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.](http://images.novusbio.com/images2/p16INK4a-CDKN2A-Antibody-Western-Blot-NB200-106-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0004.jpg)
![Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.](http://images.novusbio.com/images2/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0006.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMS2.](http://images.novusbio.com/images2/PMS2-Antibody-(OTI2G5)-Western-Blot-NBP2-46459-img0008.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/PMS2-Antibody-(OTI2G5)-Immunohistochemistry-NBP2-46459-img0007.jpg)
![Immunohistochemistry: PMS2 Antibody (OTI2G5) [NBP2-46459] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/PMS2-Antibody-(OTI2G5)-Immunohistochemistry-NBP2-46459-img0002.jpg)
![Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.](http://images.novusbio.com/images2/APC-Antibody-Western-Blot-NB100-91662-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/APC-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91662-img0006.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.](http://images.novusbio.com/images2/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0005.jpg)
![Western Blot: PMS1 Overexpression Lysate (Adult Normal) [NBP2-09011] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PMS1.](http://images.novusbio.com/images/PMS1 Overexpression Lysate (Adult Normal)-Western Blot-NBP2-09011-img0001.jpg)
![Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.](http://images.novusbio.com/images2/MGMT-Antibody-(MT-23.2)-Western-Blot-NB100-168-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT (Green) in Hela cells using NB100-168 at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/images2/MGMT-Antibody-(MT-23.2)-Immunocytochemistry-Immunofluorescence-NB100-168-img0004.jpg)
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 23.2) [NB100-168] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:2000 conc. of MGMT antibody (clone MT 23.2) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of MGMT was observed. Staining was performed by Histowiz.](http://images.novusbio.com/images2/MGMT-Antibody-(MT-23.2)-Immunohistochemistry-Paraffin-NB100-168-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Coagulation-Factor-XIV-Protein-C-Antibody-(JM93-61)-Immunocytochemistry-Immunofluorescence-NBP2-66987-img0002.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Protein C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/Coagulation-Factor-XIV-Protein-C-Antibody-(JM93-61)-Immunohistochemistry-Paraffin-NBP2-66987-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Coagulation-Factor-XIV-Protein-C-Antibody-(JM93-61)-Immunocytochemistry-Immunofluorescence-NBP2-66987-img0001.jpg)
![Western Blot: MSH3 Antibody [NBP2-19417] - H1299 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/MSH3-Antibody-Western-Blot-NBP2-19417-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MSH3 Antibody [NBP2-19417] - MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:500.Blue: Fluoroshield with DAPI.Scale bar= 10 um.](http://images.novusbio.com/images2/MSH3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19417-img0006.jpg)
![Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/images2/MSH3-Antibody-Immunohistochemistry-Paraffin-NBP2-19417-img0007.jpg)
![Western Blot: B-Raf Antibody (OTI5A9) [NBP1-47668] - Analysis of extracts (35ug) from 9 different cell lines by using anti-B Raf monoclonal antibody.](http://images.novusbio.com/images2/B-Raf-Antibody-(OTI5A9)-Western-Blot-NBP1-47668-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY B Raf.](http://images.novusbio.com/images2/B-Raf-Antibody-(OTI5A9)-Immunocytochemistry-Immunofluorescence-NBP1-47668-img0013.jpg)
![Immunohistochemistry-Paraffin: B-Raf Antibody (OTI5A9) [NBP1-47668] - Staining of paraffin-embedded Human pancreas tissue using anti-B Raf mouse monoclonal antibody.](http://images.novusbio.com/images2/B-Raf-Antibody-(OTI5A9)-Immunohistochemistry-Paraffin-NBP1-47668-img0018.jpg)
![Western Blot: SIL1 Antibody (3E3) [NBP2-02377] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIL1.](http://images.novusbio.com/images/SIL1 Antibody (3E3)-Western Blot-NBP2-02377-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SIL1 Antibody (3E3) [NBP2-02377] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.](http://images.novusbio.com/images/SIL1 Antibody (3E3)-Immunocytochemistry-Immunofluorescence-NBP2-02377-img0008.jpg)
![Immunohistochemistry-Paraffin: SIL1 Antibody (3E3) [NBP2-02377] - Staining of paraffin-embedded liver tissue using anti-SIL1 mouse monoclonal antibody.](http://images.novusbio.com/images/SIL1 Antibody (3E3)-Immunohistochemistry-Paraffin-NBP2-02377-img0007.jpg)
![Western Blot: BRCA1 Antibody (6B4) [NB100-404] - Whole cell extracts (30 and 50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody at a dilution of 1:500.](http://images.novusbio.com/images2/BRCA1-Antibody-(6B4)-Western-Blot-NB100-404-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (6B4) [NB100-404] - Staining of BRCA1 nuclear foci induced by ionizing radiation. IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining. DAPI was used to counterstain nucleus. 6B4 was used. Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4)](http://images.novusbio.com/images2/BRCA1-Antibody-(6B4)-Immunocytochemistry-Immunofluorescence-NB100-404-img0006.jpg)
![Immunohistochemistry: BRCA1 Antibody (6B4) [NB100-404]](http://images.novusbio.com/images/BRCA1-Antibody-(6B4)-Immunohistochemistry-NB100-404-img0001.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.](http://images.novusbio.com/images2/KRAS-Antibody-(3B10-2F2)-Western-Blot-H00003845-M01-img0004.jpg)
![Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/KRAS Antibody (3B10-2F2)-Western Blot-H00003845-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of monoclonal antibody to KRAS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/KRAS-Antibody-(3B10-2F2)-Immunocytochemistry-Immunofluorescence-H00003845-M01-img0006.jpg)
![Western Blot: SUB1 Antibody [NBP1-82454] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/images2/SUB1-Antibody-Western-Blot-NBP1-82454-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SUB1 Antibody [NBP1-82454] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/images2/SUB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82454-img0011.jpg)
![Immunohistochemistry-Paraffin: SUB1 Antibody [NBP1-82454] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.](http://images.novusbio.com/images2/SUB1-Antibody-Immunohistochemistry-Paraffin-NBP1-82454-img0015.jpg)
![Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.](http://images.novusbio.com/images/RASSF1 Antibody (2B11)-Western Blot-NBP2-03644-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (2B11) [NBP2-03644] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.](http://images.novusbio.com/images/RASSF1 Antibody (2B11)-Immunocytochemistry-Immunofluorescence-NBP2-03644-img0014.jpg)
![Immunohistochemistry-Paraffin: RASSF1 Antibody (2B11) [NBP2-03644] - Staining of paraffin-embedded Human prostate tissue using anti-RASSF1 mouse monoclonal antibody.](http://images.novusbio.com/images/RASSF1 Antibody (2B11)-Immunohistochemistry-Paraffin-NBP2-03644-img0013.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")