MKRN2 Antibody

Western Blot: MKRN2 Antibody [NBP2-17301] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: MKRN2 Antibody [NBP2-17301] - Immunofluorescence analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: MKRN2 Antibody [NBP2-17301] - U373 xenograft, using MKRN2 antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MKRN2 Antibody Summary

• Description: Novus Biologicals Rabbit MKRN2 Antibody (NBP2-17301) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-MKRN2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MKRN2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MKRN2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MKRN2 Antibody

• EC 6.3.2.-
• HSPC070
• makorin ring finger protein 2
• probable E3 ubiquitin-protein ligase makorin-2
• RNF62RING finger protein 62

Background

Members of the makorin family, including MKRN2, have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al., 2001 [PubMed 11597136]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MKRN2 Antibody (NBP2-17301)(2)

Publications using NBP2-17301

• Bonazza S, Coutts H, Sukumar S et al. Identifying cellular RNA-binding proteins during infection uncovers a role for MKRN2 in influenza mRNA trafficking bioRxiv 2023-10-31 (ICC/IF, WB, Human)
  
  Details:
  WB Dilution 1:5000; ICC/IF Dilution 1:200
• Bonazza S, Coutts HL, Sukumar S et Al. Identifying cellular RNA-binding proteins during infection uncovers a role for MKRN2 in influenza mRNA trafficking PLoS Pathog 2024-05-16 [PMID: 38753876]

Bioinformatics

• Gene Symbol: MKRN2
• Uniprot:
  • Q9H000(Human)