MKP-1/DUSP1 Antibody (408612) [DyLight 550]

• Reactivity: Hu
• Applications: IHC
• Clone: 408612
• Clonality: Monoclonal
• Host: Rat
• Conjugate: DyLight 550

MKP-1/DUSP1 Antibody (408612) [DyLight 550] Summary

• Immunogen: E. coli-derived recombinant human MKP-1
  Met1-Cys367
  Accession # P28562
• Specificity: Detects human MKP-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) MKP-2, rhMKP-3, or rhMKP-5 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: DUSP1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MKP-1/DUSP1 Antibody (408612) [DyLight 550]

• CL100
• CL100MAP kinase phosphatase 1
• dual specificity phosphatase 1
• Dual specificity protein phosphatase hVH1
• DUSP1
• EC 3.1.3.16
• EC 3.1.3.48
• HVH1
• Mitogen-activated protein kinase phosphatase 1
• MKP1
• MKP-1
• MKP-1dual specificity protein phosphatase 1
• Protein-tyrosine phosphatase CL100
• PTPN10
• PTPN10serine/threonine specific protein phosphatase
• VH1

Background

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DUSP1