MKP-1/DUSP1 Antibody (408612) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: IHC
• Clone: 408612
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

MKP-1/DUSP1 Antibody (408612) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human MKP-1
  Met1-Cys367
  Accession # P28562
• Specificity: Detects human MKP-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) MKP-2, rhMKP-3, or rhMKP-5 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: DUSP1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MKP-1/DUSP1 Antibody (408612) [CoraFluor™ 1]

• CL100
• CL100MAP kinase phosphatase 1
• dual specificity phosphatase 1
• Dual specificity protein phosphatase hVH1
• DUSP1
• EC 3.1.3.16
• EC 3.1.3.48
• HVH1
• Mitogen-activated protein kinase phosphatase 1
• MKP1
• MKP-1
• MKP-1dual specificity protein phosphatase 1
• Protein-tyrosine phosphatase CL100
• PTPN10
• PTPN10serine/threonine specific protein phosphatase
• VH1

Background

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DUSP1