MKK6/MEK6 Antibody (3H12C8)

Western Blot: MKK6/MEK6 Antibody (3H12C8) [NBP2-37638] - Western blot analysis using MAP2K6 mouse mAb against HepG2 (1), MCF-7 (2) and NIH/3T3 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: MKK6/MEK6 Antibody (3H12C8) [NBP2-37638] - Immunofluorescence analysis of Hela cells using MAP2K6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: MKK6/MEK6 Antibody (3H12C8) [NBP2-37638] - Flow cytometric analysis of Hela cells using MAP2K6 mouse mAb (green) and negative control (purple).

ELISA: MKK6/MEK6 Antibody (3H12C8) [NBP2-37638] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

MKK6/MEK6 Antibody (3H12C8) Summary

• Immunogen: Purified recombinant fragment of human MAP2K6 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP2K6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for MKK6/MEK6 Antibody (3H12C8)

• MAP kinase kinase 6
• MAP2K6
• MAPK/ERK kinase 6
• MAPKK6dual specificity mitogen-activated protein kinase kinase 6
• MEK6
• MEK6MAPKK 6
• mitogen-activated protein kinase kinase 6
• MKK6
• MKK6MEK 6
• PRKMK6
• PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
• SAPKK3
• SAPKK3EC 2.7.12.2

Background

Mitogen-acitvated protein kinase kinase 6 (MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK6, closely related to MKK3, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MTK1 phosphorylates MKK6 on serine 207 and threonine 211 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K6