MKK3/MEK3 Antibody

Western Blot: MKK3/MEK3 Antibody [H00005606-B01P] - Analysis of MAP2K3 expression in A-431.

Western Blot: MKK3/MEK3 Antibody [H00005606-B01P] - Analysis of MAP2K3 expression in transfected 293T cell line by MAP2K3 polyclonal antibody. Lane 1: MAP2K3 transfected lysate(38.17 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

MKK3/MEK3 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MAP2K3 (NP_659731.1, 1 a.a. - 347 a.a.) full-length human protein. MESPASSQPASMPQSKGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS
• Specificity: Reacts with mitogen-activated protein kinase kinase 3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MAP2K3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against cell and transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MKK3/MEK3 Antibody

• MAP kinase kinase 3
• MAP2K3
• MAPK/ERK kinase 3
• MAPKK 3
• MAPKK3PRKMK3dual specificity mitogen-activated protein kinase kinase 3
• MEK3
• MEK3MEK 3
• mitogen activated protein kinase kinase 3
• mitogen-activated protein kinase kinase 3
• MKK3
• MKK3EC 2.7.12.2
• PRKMK3

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K3
• Entrez:
  • 5606(Human)
• Uniprot:
  • NP_659731.1(Human)