MKK3/MEK3 Antibody (SD20-93)

Western Blot: MKK3/MEK3 Antibody (SD20-93) [NBP2-67747] - Analysis of MEK3 on Hela lysates using anti-MEK3 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (SD20-93) [NBP2-67747] - Analysis of paraffin-embedded mouse spleen tissue using anti-MEK3 antibody. Counter stained with hematoxylin.

Flow Cytometry: MKK3/MEK3 Antibody (SD20-93) [NBP2-67747] - Flow cytometric analysis of Hela cells with MKK3/MEK3 antibody at 1/50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (SD20-93) [NBP2-67747] - Analysis of Hela cells with MEK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (SD20-93) [NBP2-67747] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (SD20-93) [NBP2-67747] - Analysis of paraffin-embedded human liver tissue using anti-MEK3 antibody. Counter stained with hematoxylin.

Product Discontinued

MKK3/MEK3 Antibody (SD20-93) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within human MKK3/MEK3 aa 101-347/347. (SwissProt: P46734 Human; SwissProt: O09110 Mouse; Unigene: 100064 Rat)
• Localization: Cytosol, nucleoplasm, membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP2K3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MKK3/MEK3 Antibody (SD20-93)

• MAP kinase kinase 3
• MAP2K3
• MAPK/ERK kinase 3
• MAPKK 3
• MAPKK3PRKMK3dual specificity mitogen-activated protein kinase kinase 3
• MEK3
• MEK3MEK 3
• mitogen activated protein kinase kinase 3
• mitogen-activated protein kinase kinase 3
• MKK3
• MKK3EC 2.7.12.2
• PRKMK3

Background

Mitogen-acitvated protein kinase kinase 3 (MKK3) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK3, closely related to MKK6, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MKK3 is activated by mitogenic and environ-mental stress stimulus as well as by insulin. MKK3 is necessary for the expression of glucose transporter and participates in the MAP kinase- mediated signaling cascade (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K3