Mitofusin 2 Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Mitofusin 2 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human Mitofusin 2
  Arg364-Phe599
  Accession # O95140
• Specificity: Detects human, mouse, and rat Mitofusin 2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human Mitofusin 1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mitofusin 2 Antibody [Alexa Fluor® 750]

• CMT2A
• CMT2A2
• CPRP1
• CPRP1mitochondrial assembly regulatory factor
• EC 3.6.5
• EC 3.6.5.-
• HSG
• KIAA0214hyperplasia suppressor
• MARF
• MFN2
• Mitofusin 2
• mitofusin-2
• Transmembrane GTPase MFN2

Background

MFN-2 (Mitofusin 2; also Hypertension-related protein 1, Mitochondrial assembly regulatory factor, HSG protein, and Transmembrane GTPase MFN-2) is an 85‑100 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. Through trans-interactions with MFN-1 and/or MFN-2, MFN-2 apparently mediates the fusion of individual mitochondria with either the ER or with adjacent mitochondria. Pertubations with this process result in both a general failure of mitochondria to fuse, and in cell-specific effects such as a reduction in oxidative phosphorylation, a decrease in the axonal transport of mitochondria, and a deficit in the expression of respiratory chain components. MFN-2 has two key domains. One is a coiled-coil region that mediates MFN-2:MFN-1/2 binding, and a second is a GTPase domain that likely plays a role in fusion. While active, the MFN-2 GTPase domain is 8-fold lower in activity than that for MFN-1. Human MFN-2 is a 757 amino acid (aa) two transmembrane protein that contains a cytoplasmic N- and C‑terminus. The cytoplasmic N‑terminus (aa 1-604) contains the GTPase domain (aa 99-258) while the cytoplasmic C‑terminus possesses a critical coiled-coil motif (aa 696-738). MFN-2 is known to form oligomers, either with itself or MFN-1. Two potential splice variants are reported, one that shows a deletion of aa 245-273, and a second that contains a 33 aa substitution for aa 573-757, suggesting this might be a soluble for of MFN-2. Over aa 364-599, human and mouse MFN-2 share 93% aa sequence identity. Full-length human MFN-2 and MFN-1 share 61% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.