MIS RII/AMHR2 Antibody

• Reactivity: Rt
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MIS RII/AMHR2 Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat MIS RII
  Pro19-Pro144
  Accession # Q62893
• Specificity: Detects rat MIS RII in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TGF‑ beta RII, rhTGF-beta RIII, recombinant mouse (rm) TGF-beta RI, and rmTGF-beta RII is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AMHR2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIS RII/AMHR2 Antibody

• AMH type II receptor
• AMHR2
• AMHREC 2.7.11.30
• AMHRII
• C14
• MIS RII
• MISR2
• MISRII
• MISRIIMIS type II receptor
• MRII
• Muellerian hormone type II receptor
• Muellerian hormone type-2 receptor
• Mullerian hormone receptor, type II
• Mullerian inhibiting substance type II receptor

Background

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH) is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to fetal testis, plus postnatal testis and ovary (1). MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo and has been shown to have a regulatory role in gonads postnatally (1). Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, can be utilized for MIS signaling (1). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members (1, 2). Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad8 (1).

The gene for rat MIS RII was isolated separately by two groups working from Sertoli cell and fetal ovary cDNA libraries (3, 4). MIS RII comprises a 557 amino acid (aa) residue type I transmembrane protein with a putative 17 aa signal peptide. Mature MIS RII has a 127 aa cysteine-rich extracellular domain containing 2 potential N-glycosylation sites, a 21 aa transmembrane domain, and a 392 aa cytoplasmic region with a serine/threonine kinase domain (3, 4). Rat MIS RII shares 95% and 82% aa sequence identity with the mouse and human homologues, respectively (5). MIS RII is expressed in the mesenchymal cells surrounding the Mullerian ducts during embryonic development. Postnatally, it is expressed in uterine tissues and rodent Leydig cells, and coexpressed with MIS in the testicular Sertoli and ovarian granulosa cells (1, 6). The expression of MIS RII in the Mullerian mesenchyme is regulated by Wnt7a signaling from nearby epithelium through the canonical Wnt pathway. Wnt7a mutant mice do not express MIS RII, and do not experience Mullerian duct regression (7).

1. Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
2. Mishna, Y. et al. (1999) Endocrinology 140:2084.
3. Baarends, W. et al. (1994) Development 120:189.
4. di Clement, N. et al. (1994) Mol. Endocrinol. 8:1006.
5. Mishina, Y. et al. (1997) Biochem. Biophys. Res. Comm. 237:741.
6. Teixeira, J. et al. (1996) Endocrinology 137:160.
7. Hossain, A. and G. Saunders (2003) J. Biol. Chem. 278:26511.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIS RII/AMHR2 Antibody (AF1618)(4)

Publications using AF1618

• MC Meinsohn, CHK Hughes, A Estienne, HD Saatcioglu, D Pépin, R Duggavathi, BD Murphy A role for orphan nuclear receptor liver receptor homolog-1 (LRH-1, NR5A2) in primordial follicle activation Scientific Reports, 2021;11(1):1079. 2021 [PMID: 33441767] (ICC, Mouse)
• SA Malone, GE Papadakis, A Messina, NEH Mimouni, S Trova, M Imbernon, C Allet, I Cimino, J Acierno, D Cassatella, C Xu, R Quinton, G Szinnai, P Pigny, L Alonso-Cot, L Masgrau, JD Maréchal, V Prevot, N Pitteloud, P Giacobini Defective AMH signaling disrupts GnRH neuron development and function and contributes to hypogonadotropic hypogonadism Elife, 2019;8(0):. 2019 [PMID: 31291191] (Neutralization, Mouse)
• G Garrel, C Racine, D L'Hôte, C Denoyelle, CJ Guigon, N di Clement, J Cohen-Tann Anti-M�llerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty Sci Rep, 2016;6(0):23790. 2016 [PMID: 27030385] (Western Blot, Mouse)
• Wang PY, Protheroe A, Clarkson AN, Imhoff F, Koishi K, McLennan IS Mullerian inhibiting substance contributes to sex-linked biases in the brain and behavior. Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7203-8. 2009 [PMID: 19359476] (IHC, Mouse)

Bioinformatics

• Gene Symbol: AMHR2
• Uniprot:
  • Q62893()