Mind Bomb 2/MIB2 Antibody (3A7) - Azide and BSA Free

Sandwich ELISA: Mind Bomb 2/MIB2 Antibody (3A7) [H00142678-M02] - Detection limit for recombinant GST tagged MIB2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: ELISA
• Clone: 3A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Mind Bomb 2/MIB2 Antibody (3A7) - Azide and BSA Free Summary

• Immunogen: MIB2 (AAH37542, 381 a.a. ~ 480 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRL
• Specificity: MIB2 - mindbomb homolog 2 (Drosophila) (3A7)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MIB2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
• Application Notes: This product is useful for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mind Bomb 2/MIB2 Antibody (3A7) - Azide and BSA Free

• E3 ubiquitin-protein ligase MIB2
• EC 6.3.2
• EC 6.3.2.-
• EC 6.3.2.13
• FLJ20648
• FLJ41406
• MIB2
• Mind Bomb 2
• Mind bomb homolog 2
• Mind Bomb-2
• mindbomb homolog 2 (Drosophila)
• Novel zinc finger protein
• Novelzin
• Putative NF-kappa-B-activating protein 002N
• SKD
• Skeletrophin
• Zinc finger ZZ type with ankyrin repeat domain protein 1
• ZZ type with ankyrin repeat domain 1
• ZZANK1
• ZZZ5

Background

MIB2/Skeletrophin is an actin-binding protein that bears two MIB/HERC2 domains, a ZZ-type zinc-finger domain, nine ankyrin repeats, and two RING-finger domains. The C-terminal RING domain has been found to have E3 ubiquitin ligase activity that mediates ubiquitination of the intracellular regions of the Notch receptor and its ligands to regulate Notch signaling. MIB2/Skeletrophin appears to be important to muscle development and may function as a suppressor of melanoma invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MIB2
• Entrez:
  • 142678(Human)
• Uniprot:
  • AAH37542(Human)