MIG2/Kindlin-2 Antibody (696906)

Western blot shows lysates of HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MIG2 Monoclonal Antibody (Catalog # MAB6984) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MIG2 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 696906
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MIG2/Kindlin-2 Antibody (696906) Summary

• Immunogen: E. coli-derived recombinant human MIG2
  Gln321-Leu444
  Accession # Q96AC1
• Specificity: Detects human MIG2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MIG, rhFERMT1 (aa 514‑677), rhMIG2/FERMT2 (aa 514‑680), or rhFERMT3 (498‑667) is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FERMT2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIG2/Kindlin-2 Antibody (696906)

• fermitin family homolog 2 (Drosophila)
• fermitin family homolog 2
• fermitin family member 2
• FERMT2
• KIND2
• KIND2DKFZp686G11125
• kindlin 2
• Kindlin-2
• MIG2
• mig-2
• MIG2FLJ34213
• mitogen inducible gene 2 protein
• Mitogen-inducible gene 2 protein
• PH domain-containing family C member 1
• pleckstrin homology domain containing, family C (with FERM domain) member 1
• pleckstrin homology domain containing, family C member 1
• Pleckstrin homology domain-containing family C member 1
• PLEKHC1
• PLEKHC1FLJ44462
• UNC112
• UNC112B

Background

MIG2 (mitogen-inducible gene-2), also known as FERMT2 or Kindlin-2, is an  approximately 80 kDa, 680 amino acid (aa), ubiquitously expressed member of the Fermitin/Kindlin family of cytoplasmic adaptor proteins. MIG2 is essential for beta 1 and beta 3 integrin activity due to its interactions with regulatory molecules such as talin and ILK. MIG2 contains two FERM domains (aa 285-326 and aa 524-573) and one pleckstrin homology domain (aa 373-478). Within the region used as an immunogen, human MIG2 shares 98% aa identity with mouse and rat MIG2. Two isoforms (687 aa and 633 aa) have a 7 aa insert after aa 534. The shorter isoform also has a 2 aa substitution for the C-terminal 57 aa of the 680 aa form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FERMT2
• Uniprot:
  • Q96AC1()