MHC Class II Antibody (CA2.1C12)

Product Discontinued

MHC Class II Antibody (CA2.1C12) Summary

• Immunogen: Made to Dog MHC CLASS II MONOMORPHIC
• Specificity: Recognizes a monomorphic epitope on canine MHC Class II which was classified at the First Canine Leucocyte Antigen Workshop (CLAW) [Cobbold et al. 1992]. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DMA
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Neat
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Reacts with Canine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue Culture Supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for MHC Class II Antibody (CA2.1C12)

• class II histocompatibility antigen, M alpha chain
• D6S222E
• DMA
• HLA class II histocompatibility antigen, DM alpha chain
• major histocompatibility complex, class II, DM alpha
• MHC class II antigen DMA
• Really interesting new gene 6 protein
• RING6HLADM

Background

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DMA
• Entrez:
  • 3108(Human)
• Uniprot:
  • P28067(Human)