MGST3 Antibody

Western Blot: MGST3 Antibody [NBP2-75572] - Analysis of GST3 on different cell lysate using anti-GST3 antibody at 1/1,000 dilution.Positive control:Lane1: Siha Lane2: HelaLane3: A549 Lane4: Mouse kidney tissueLane5: Rat liver tissue

Immunocytochemistry/Immunofluorescence: MGST3 Antibody [NBP2-75572] - Staining GST3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MGST3 Antibody [NBP2-75572] - Analysis of paraffin-embedded human placenta tissue using anti-GST3 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: MGST3 Antibody [NBP2-75572] - Staining GST3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MGST3 Antibody [NBP2-75572] - Staining GST3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MGST3 Antibody [NBP2-75572] - Analysis of paraffin-embedded human liver cancer tissue using anti-GST3 antibody. Counter stained with hematoxylin.

Product Discontinued

MGST3 Antibody Summary

• Immunogen: Recombinant protein within N-terminal human MGST3. (SwissProt: P09211 Human; SwissProt: P19157 Mouse; SwissProt: P04906 Rat)
• Localization: Nucleus. Cytoplasm. Mitochondrion.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MGST3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MGST3 Antibody

• EC 2.5.1.18
• GST-III
• microsomal glutathione S-transferase 3
• microsomal glutathione S-transferase III
• Microsomal GST-3
• Microsomal GST-III

Background

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, several of which are involved the production of leukotrienes and prostaglandin E, important mediators of inflammation. This gene encodes an enzyme which catalyzes the conjugation of leukotriene A4 and reduced glutathione to produce leukotriene C4. This enzyme also demonstrates glutathione-dependent peroxidase activity towards lipid hydroperoxides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MGST3