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Human MGST2 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Standard Curve Range
78.125 - 5000 pg/mL (example only; lot dependent)
Sensitivity
25.5 pg/mL (example only; lot dependent)

Order Details

Human MGST2 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
78.125 - 5000 pg/mL (example only; lot dependent)
Sensitivity
25.5 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MGST2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Alternate Names for Human MGST2 ELISA Kit (Colorimetric)

  • EC 2.5.1.18
  • FLJ27438
  • GST2MGC14097
  • MGST-II
  • microsomal glutathione S-transferase 2
  • Microsomal GST-2
  • Microsomal GST-II
  • truncated microsomal glutathione S-transferase 2

Background

Characterized as important mediators of inflammation, MGST2 (microsomal glutathione S-transferase 2) is an important regulator of leukotriene and glutathione. MGST2 belongs to the MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family of six human proteins. Several of these proteins are involved in the creation of leukotrienes and prostaglandin E. MGST2 catalyzes the production of LTC4 from LTA4 and reduced glutathione, and also catalyzes the conjugation of 1-chloro-2, 4-dinitrobenzene with reduced glutathione. Diseases associated with this gene include psoriasis, asthma, leukemia, prostatitis, breast cancer and progressive familial intrahepatic cholestasis type 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for MGST2 ELISA Kit (NBP3-54993). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol MGST2