MGMT Antibody (MT 3.1)

Images

 
Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & ...read more
Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin fixed paraffin embedded human tonsil stained with MGMT antibody.
Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MGMT MT 3.1 (Cat. #NB 100-692) using peroxidase-conjugate and AEC chromogen. Note ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
MT 3.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MGMT Antibody (MT 3.1) Summary

Immunogen
Recombinant human MGMT protein.
Localization
Nuclear and cytoplasmic.
Specificity
This stains all mantle zone lymphocytes and 50% of germinal center lymphocytes. Basaloid epithelial cells of tonsil squamous mucosa were also stained positive with this.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MGMT
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Paraffin 1:25-1:50
  • Western Blot 1:100-1:2000
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in western blot reported in scientific literature (PMID 24019948).
Control
Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB100-692 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    3 publications

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS (pH 7.4), 0.2% BSA, Tween-20
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for MGMT Antibody (MT 3.1)

  • 6-O-methylguanine-DNA methyltransferase
  • EC 2.1.1.63
  • methylated-DNA--protein-cysteine methyltransferase
  • methylguanine-DNA methyltransferase
  • MGMT
  • O-6-methylguanine-DNA methyltransferase
  • O6-methylguanine-DNA methyltransferase
  • O-6-methylguanine-DNA-alkyltransferase

Background

O6-Methylguanine-DNA-Methyltransferase (MGMT) is a 22 kD human DNA repair protein that removes O6-alkylguanine DNA adducts. MGMT acts as a suicide acceptor protein repairing the DNA by accepting alkyl groups and consequently inactivating itself. This antibody stains all mantle zone lymphocytes and 50%of germinal center lymphocytes. Basaloid epithelial cells of tonsil squamous mucosa were also stained positive with this antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MGMT Antibody (NB100-692)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-692 Applications Species
Ishida A, Shichi H, Fukuoka H Et al. Efficacy of temozolomide combined with capecitabine (CAPTEM) on refractory prolactinomas as assessed using an ex vivo 3D spheroid assay Pituitary Nov 13 2021 [PMID: 34773564]
Rabe M, Dumont S, Alvarez-Arenas A et al. Identification of a transient state during the acquisition of temozolomide resistance in glioblastoma Cell Death Dis Jan 6 2020 [PMID: 31907355] (WB, Human) WB Human
Palmieri D, Duchnowska R, Woditschka S et al. Profound prevention of experimental brain metastases of breast cancer by temozolomide in an MGMT-dependent manner. Clin. Cancer Res. 2014 Mar 14 [PMID: 24634373] (IHC-P, WB, Human)

Details:
IHC (P): mouse brain (Fig 1E), human primary breast tumors and brain metastases (Fig 4, Tables 1,2,S1). Nuclear staining was considered positive. Tonsil tissue was used as a positive control. WB: cell lines transfected with human MGMT (Figs 1B, S2C. The s
IHC-P, WB Human
Gupte M, Tuck AN, Sharma VP, Williams KJ. Major Differences between Tumor and Normal Human Cell Fates after Exposure to Chemotherapeutic Monofunctional Alkylator. PLoS One. 2013 Sep 3 [PMID: 24019948] (WB, Human) WB Human
Zuhur SS, Tanik C, Karaman O et al. MGMT immunoexpression in growth hormone-secreting pituitary adenomas and its correlation with Ki-67 labeling index and cytokeratin distribution pattern Endocrine 2011 Oct [PMID: 21792693] (IHC, Human) IHC Human
Zuhur SS, Musluman AM, Tan?k C, Karaman O, Ozturk FY, Ozderya A, Ozkayalar H, Ayd?n Y, Altunta? Y. MGMT immunoexpression in adamantinomatous craniopharyngiomas. Pituitary;14(4):323-7. 2011 Dec. [PMID: 21318329] (IHC, Human) IHC Human
Takeshita A, Inoshita N, Taguchi M et al. High incidence of low O6-methylguanine DNA methyltransferase expression in invasive macroadenomas of Cushing's disease. Eur J Endocrinol;161(4):553-559. 2009 [PMID: 19589911]

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Control Lysate(s)

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Bioinformatics

Gene Symbol MGMT
Entrez
Uniprot