MGAT4B Overexpression Lysate (Native)

Images

 
Western Blot: MGAT4B Overexpression Lysate (Adult Normal) [NBL1-13055] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MGAT4B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MGAT4B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208670 and based on accession number NM_014275. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B (MGAT4B), transcript variant 1, mRNA.
Gene
MGAT4B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MGAT4B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MGAT4B Overexpression Lysate (Native)

  • alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
  • alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
  • aminyltransferase
  • EC 2.4.1.145
  • GlcNAc-T IVb
  • GNT-IV
  • GNT-IVB
  • isoenzyme B
  • isozyme B
  • mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
  • N-acetylglucosaminyltransferase IVb
  • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb
  • UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IV
  • UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IVb

Background

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1, 4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq]. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MGAT4B