MGAT4B Antibody (1F4)

Western Blot: MGAT4B Antibody (1F4) [H00011282-M01] - MGAT4B monoclonal antibody (M01), clone 1F4. Analysis of MGAT4B expression in human pancreas.

Immunocytochemistry/Immunofluorescence: MGAT4B Antibody (1F4) [H00011282-M01] - Analysis of monoclonal antibody to MGAT4B on HeLa cell . Antibody concentration 10 ug/ml.

Product Discontinued

MGAT4B Antibody (1F4) Summary

• Immunogen: MGAT4B (NP_055090, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AEVSTSLKTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGY
• Specificity: MGAT4B - mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MGAT4B
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactive against recombinant protein on ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MGAT4B Antibody (1F4)

• alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
• alpha-1,3-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• aminyltransferase
• EC 2.4.1.145
• GlcNAc-T IVb
• GNT-IV
• GNT-IVB
• isoenzyme B
• isozyme B
• mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• N-acetylglucosaminyltransferase IVb
• N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVb
• UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IV
• UDP-N-acetylglucosamine: alpha-1,3-D-mannosidebeta-1,4-N-acetylglucosaminyltransferase IVb

Background

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MGAT4B Antibody (H00011282-M01)(2)

Publications using H00011282-M01

• Maszczak-Seneczko D, Sosicka P, Olczak T, Olczak M. In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures. Methods Mol Biol 2016-09-06 [PMID: 27632007]
• Kippe JM, Mueller TM, Haroutunian V, Meador-Woodruff JH. Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia Schizophr. Res. 2015-06-20 [PMID: 26104473] (WB, Human)

Bioinformatics

• Gene Symbol: MGAT4B
• Entrez:
  • 11282(Human)
• OMIM:
  • 604561(Human)
• Uniprot:
  • NP_055090(Human)