![Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-212024939189.jpg "Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1816 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit METTL14 Antibody (HL1816) - Azide and BSA Free (NBP3-25562) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human METTL14. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | METTL14 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for METTL14 Antibody (NBP3-25562)Find related products by research area.
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| Gene Symbol | METTL14 |
![Immunocytochemistry/Immunofluorescence: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - METTL14 antibody [HL1816] detects METTL14 protein by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: METTL14 stained by METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-2120249355576.jpg "Immunocytochemistry/Immunofluorescence: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - METTL14 antibody [HL1816] detects METTL14 protein by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: METTL14 stained by METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
![Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-2120249351395.jpg "Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - METTL14 antibody [HL1816] detects METTL14 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. METTL14 stained by METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-2120249435519.jpg "Immunohistochemistry-Paraffin: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - METTL14 antibody [HL1816] detects METTL14 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. METTL14 stained by METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Untransfected (-) and transfected (+) A549 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-2120249362792.jpg "Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Untransfected (-) and transfected (+) A549 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - METTL14 antibody [HL1816] detects METTL14 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: METTL14 stained by METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-212024939110.jpg "Immunocytochemistry/Immunofluorescence: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - METTL14 antibody [HL1816] detects METTL14 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: METTL14 stained by METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-2120249413377.jpg "Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Various whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-2120249413387.jpg "Western Blot: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - Various whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - METTL14 antibody [HL1816] detects METTL14 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. METTL14 stained by METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25562_rabbit-mettl14-mab-hl1816-azide-and-bsa-free-2120249415969.jpg "Immunohistochemistry-Paraffin: METTL14 Antibody (HL1816) - Azide and BSA Free [NBP3-25562] - METTL14 antibody [HL1816] detects METTL14 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. METTL14 stained by METTL14 antibody [HL1816] (NBP3-25562) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")