METTL1 Antibody - Azide and BSA Free

Western Blot: METTL1 Antibody [NBP2-99811] - Anti-METTL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).

Immunohistochemistry-Paraffin: METTL1 Antibody [NBP2-99811] - Immunochemical staining of human METTL1 in human bladder carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated wit

Immunoprecipitation: METTL1 Antibody [NBP2-99811] - METTL1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate0.5 ul anti-METTL1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-METTL1 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

METTL1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human METTL1 (Accession#: NP_005362.3; Asp 32-Gln 265)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: METTL1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for METTL1 Antibody - Azide and BSA Free

• C12orf1
• D1075-like gene product
• EC 2.1.1.33
• FLJ95748
• methyltransferase like 1
• methyltransferase-like 1
• Methyltransferase-like protein 1
• TRM8
• tRNA (guanine-N(7)-)-methyltransferase
• tRNA(m7G46)-methyltransferase
• YDL201w

Background

METTL1 is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: METTL1