WDR4 Antibody (OTI4G1) - Azide and BSA Free

Western Blot: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR4.

Immunocytochemistry/Immunofluorescence: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY WDR4.

Flow Cytometry: WDR4 Antibody (OTI4G1) - Azide and BSA Free [NBP2-74865] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-WDR4 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI4G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

WDR4 Antibody (OTI4G1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse WDR4 Antibody (OTI4G1) - Azide and BSA Free (NBP2-01409) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human WDR4(NP_387510) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: WDR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 45.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for WDR4 Antibody (OTI4G1) - Azide and BSA Free

• tRNA (guanine-N(7)-)-methyltransferase subunit WDR4
• WD repeat domain 4, 10TRM82
• WD repeat-containing protein 4

Background

WDR4 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WDR4