Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79)

Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Analysis of Methionine Aminopeptidase 2/METAP2/METAP2 on Hela cell using anti-Methionine Aminopeptidase 2/METAP2/METAP2 antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: K562 Lane 3: Mouse thymus Lane 4: Mouse kidney

Immunocytochemistry/Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Staining Methionine Aminopeptidase 2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Analysis in mouse testis tissue using Methionine Aminopeptidase 2/METAP2/METAP2 antibody. Counter stained with hematoxylin.

Flow Cytometry: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Analysis of Daudi cells with Methionine Aminopeptidase 2/METAP2 antibody at 1/100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Staining Methionine Aminopeptidase 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Staining Methionine Aminopeptidase 2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Analysis in rat seminal vesicle tissue using Methionine Aminopeptidase 2/METAP2/METAP2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Analysis in human tonsil tissue using Methionine Aminopeptidase 2/METAP2/METAP2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Analysis in human prostate cancer tissue using Methionine Aminopeptidase 2/METAP2/METAP2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) [NBP2-80467] - Analysis in human kidney tissue using Methionine Aminopeptidase 2/METAP2/METAP2 antibody. Counter stained with hematoxylin.

Product Discontinued

Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human Methionine Aminopeptidase 2/METAP2 aa 340-478 / 478. (SwissProt: P50579 Human; SwissProt: O08663 Mouse; SwissProt: P38062 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: METAP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Methionine Aminopeptidase 2/METAP2 Antibody (JG39-79)

• Amp2
• eIF-2-associated p67 homolog
• Initiation factor 2-associated 67 kDa glycoprotein
• MAP 2
• MAP2
• MetAP 2
• METAP2
• Methionine Aminopeptidase 2
• methionyl aminopeptidase 2
• MNPEP
• p67
• p67EC 3.4.11.18
• p67eIF2
• Peptidase M 2

Background

METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: METAP2