Meteorin-like/METRNL Antibody

Meteorin-like/METRNL was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Meteorin-like/METRNL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6679) at 0.6 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Meteorin-like/METRNL Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Meteorin-like/METRNL
  Accession # Q8VE43
• Specificity: Detects mouse Meteorin-like/METRNL in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Meteorin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: METRNL
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Meteorin-like/METRNL Antibody

• Meteorin like
• meteorin, glial cell differentiation regulator-like
• meteorin-like protein
• Meteorin-like
• METRNL
• MGC99788

Background

Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1‑3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2‑5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).

1. Park, J.A. et al. (2008) Glia 56:247.
2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
4. Nishino, J. et al. (2004) EMBO J. 23:1998.
5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
6. Accession # Q8VE43.
7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.
8. Surace, C. et al. (2009) Clin. Genet. 76:256.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Meteorin-like/METRNL Antibody (AF6679)(1)

Publication using AF6679

• YW Dai, JK Ma, R Jiang, XL Zhan, SY Chen, LL Feng, Q Zhang, TB Liang, K Lv, GJ Yang, JF Lu, J Chen, XJ Lu Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization Cell Reports, 2022-09-20;40(12):111361. 2022-09-20 [PMID: 36130501] (ChIP, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: METRNL
• Uniprot:
  • Q8VE43()