Metadherin Antibody [Alexa Fluor® 405]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

Metadherin Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant mouse Metadherin
  Lys167-Ser297
  Accession # Q80WJ7
• Specificity: Detects human and mouse Metadherin in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Metadherin Antibody [Alexa Fluor® 405]

• 3D3
• AEG1
• AEG-13D3/LYRIC
• AEG1LYRIC/3D3
• Astrocyte elevated gene-1 protein
• LYRIC
• LYRICMetastasis adhesion protein
• Lysine-rich CEACAM1 co-isolated protein
• Metadherin
• MTDH
• protein LYRIC

Background

MTDH (Metadherin; also Lyric and Astrocyte-elevated gene-1/AEG1) is a unique molecule originally discovered to be upregulated in astrocytes following HIV infection. Although its predicted MW is 64 kDa, it runs anomalously at approximately 80 kDa in SDS-Page. It is widely expressed, and appears to be a component of the ER, nucleolus and inner nuclear membrane. MTDH is reported to promote Akt/PI3 kinase activity, and suppress apoptosis-associated FOXO3A transcription. Mouse MTDH is a type III (i.e.- a type I with no signal sequence) transmembrane protein 579 amino acids (aa) in length. It contains a luminal N-terminus (aa 1-48) with a 510 aa cytoplasmic C-terminus. There are no identifiable structural motifs, although two poly-Lys segments and two utilized Ser phosphorylation sites exist. Multiple bands at 80 kDa, 75 kDa, 50-55 kDa and 37 kDa are seen for MTDH on SDS-Page. They may reflect the presence of at least two potential isoform variants that show 1) a deletion of aa 422-457, and 2) an Ile substitution for aa 190-579. Over aa 167-297, mouse MTDH shares 98% and 97% aa sequence identity with rat and human MTDH, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.