MEST Antibody

Western Blot: MEST Antibody [NBP1-44050] - NBP1-44050 (0.2ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MEST Antibody Summary

• Description: Novus Biologicals Goat MEST Antibody (NBP1-44050) is a polyclonal antibody validated for use in WB and ELISA. Anti-MEST Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QELLYRYKQNRSGR corresponding to internal region according to NP_803490.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize both reported isoforms (NP_002393.2; NP_803490.1). Reported variants NP_803490.1 and NP_803491.1 represent identical protein.
• Predicted Species: Human (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MEST
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.2 - 0.5 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in mouse and rat testis lysates (calculated MW of 37.6 kDa band according to human NP_803490.1 and 38.8 kDa band according to rat NP_001009617.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MEST Antibody

• DKFZp686L18234
• EC 3.-
• mesoderm specific transcript (mouse) homolog
• mesoderm specific transcript homolog (mouse)
• mesoderm-specific transcript homolog protein
• MGC111102
• MGC8703
• paternally expressed gene 1
• PEG1Paternally-expressed gene 1 protein

Background

MEST encodes a member of the [alpha]/[beta] hydrolase fold family and has isoform specific imprinting. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Three transcript variants encoding two distinct isoforms have been identified for this gene. A pseudogene for this locus is located on chromosome 6. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEST Antibody (NBP1-44050)(1)

Publication using NBP1-44050

• Li T, Vu TH, Lee KO et al. An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa. J Biol Chem 2002-04-19 [PMID: 11821432]

Bioinformatics

• Gene Symbol: MEST
• Entrez:
  • 4232(Human)
  • 17294(Mouse)
  • 58827(Rat)
• Uniprot:
  • NP_002393.2(Human)