MEPE/OF45 Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

MEPE/OF45 Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MEPE/OF45
  Ala17-Asp433
  Accession # AAK70342
• Specificity: Detects mouse MEPE/OF45 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human MEPE/OF45 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEPE/OF45 Antibody [Alexa Fluor® 647]

• extracellular phosphoglycoprotein with ASARM motif (bone)
• matrix extracellular phosphoglycoprotein
• MEPE
• OF45
• Osteoregulin

Background

MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56‑58 kDa member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2 kDa ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417 amino acids (aa) in length. It contains an RGD motif and ASARM domain (aa 416‑433). In addition to its a 57 kDa form, 70 kDa, 42‑45 kDa and 30‑35 kDa forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa 147‑433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.