Melanotransferrin/CD228/MFI2 Antibody (893416) [Unconjugated]

A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Melanotransferrin/CD228 Monoclonal Antibody (Catalog # MAB81751, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 893416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Melanotransferrin/CD228/MFI2 Antibody (893416) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human Melanotransferrin/CD228
  Gly20-Gly711
  Accession # P08582
• Specificity: Detects human Melanotransferrin/CD228 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MFI2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Melanotransferrin/CD228/MFI2 Antibody (893416) [Unconjugated]

• antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5
• CD228 antigen
• CD228
• FLJ38863
• MAP97
• MAP97Melanoma-associated antigen p97
• Melanotransferrin
• membrane-bound transferrin-like protein
• MFI2
• MGC4856
• MTF
• MTF1
• p97

Background

Melanotransferrin, also known as MTF, CD228, Melanoma-associated Antigen p97, MAP97 and MFI2, is a 90-97 kDa sialoglycoprotein member of the transferrin family. Unlike other transferrins, which are secreted, MTF is usually found tethered to the cell membrane by a glycosyl phosphatidyl inositol anchor, with only small amounts of soluble protein detected. MTF is highly expressed on melanoma cells, and at lower levels in salivary gland, pancreas, kidney and testis. Like other transferrins, MTF is an iron-binding protein, and may play roles in cellular proliferation, tumorigenesis, metastasis and migration. Full-length human, mouse and rat MTF is 738 amino acids (aa). Over aa 1-711, human MTF shares 85 and 86% aa identity with mouse and rat MTF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MFI2
• Uniprot:
  • P08582()