MEKK3 Antibody

Western Blot: MEKK3 Antibody [H00004215-B02P] - Analysis of MAP3K3 expression in transfected 293T cell line by MAP3K3 polyclonal antibody. Lane 1: MAP3K3 transfected lysate(9.9 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

MEKK3 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: MAP3K3 (AAH10464.1, 1 a.a. - 90 a.a.) full-length human protein. MNEANVMLPYSGKEEPVLPVAMTLPLPGRGPRCGTAATEGGSSFVNAVVSVLQVGVTLMLYPVSKLETVCALWALSTPALGLGLGCIEKS
• Specificity: MAP3K3 - mitogen-activated protein kinase kinase kinase 3,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MAP3K3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MEKK3 Antibody

• EC 2.7.11.25
• MAP/ERK kinase kinase 3
• MAP3K3
• MAPK/ERK kinase kinase 3
• MAPKKK3
• MAPKKK3MEKK 3
• MEK kinase 3
• MEKK3
• mitogen-activated protein kinase kinase kinase 3

Background

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K3
• Entrez:
  • 4215(Human)