MEKK2 Antibody (722728) [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 722728
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

MEKK2 Antibody (722728) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human MEKK2
  Asn42-Tyr264
  Accession # Q9Y2U5
• Specificity: Detects recombinant human MEKK2 in direct ELISA and human, mouse, rat MEKK2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MEKK1, 3, or 4 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MEKK2 Antibody (722728) [Alexa Fluor® 750]

• EC 2.7.11.25
• MAP/ERK kinase kinase 2
• MAP3K2
• MAPK/ERK kinase kinase 2
• MAPKKK2
• MEK kinase 2
• MEKK 2
• MEKK2
• MEKK2B
• mitogen-activated protein kinase kinase kinase 2

Background

MAPK/ERK kinase kinase 2 (MEKK2) is a widely expressed 70 kDa member of the MEKK/STE11 subfamily of Ser/Thr kinases. It contains a PB1 domain (aa 43-122) which mediates dimerization with MEK5, a region with several phosphorylation sites, and a protein kinase domain (aa 357-617) that includes a site for activating phosphorylation at Thr524. Within aa 42-264, human MEKK2 shares 93% aa sequence identity with mouse and rat MEKK2. Activated MEKK2 translocates from the cytosol to the nucleus, where it forms a complex that binds and activates MKK7 in the c-Jun N-terminal kinase (JNK) pathway, relaying mitogenic and stress signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.