![Western Blot: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of MEF2A on rat spleen tissue lysate using anti-MEF2A antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/MEF2A Antibody (JA33-04)-Western Blot-NBP2-66943-img0007.jpg "Western Blot: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of MEF2A on rat spleen tissue lysate using anti-MEF2A antibody at 1/1,000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clone | JA33-04 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | C terminal Human MEF2A |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MEF2A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for MEF2A Antibody (NBP2-66943)Discover more about diseases related to MEF2A Antibody (NBP2-66943).
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| Gene Symbol | MEF2A |
![Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/MEF2A-Antibody-(JA33-04)-Immunohistochemistry-Paraffin-NBP2-66943-img0006.jpg "Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded human spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/MEF2A-Antibody-(JA33-04)-Immunohistochemistry-Paraffin-NBP2-66943-img0001.jpg "Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded human spleen tissue using anti-MEF2A antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded human tonsil tissue using anti-MEF2A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/MEF2A-Antibody-(JA33-04)-Immunohistochemistry-Paraffin-NBP2-66943-img0002.jpg "Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded human tonsil tissue using anti-MEF2A antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse cerebral cortex tissue using anti-MEF2A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/MEF2A-Antibody-(JA33-04)-Immunohistochemistry-Paraffin-NBP2-66943-img0003.jpg "Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse cerebral cortex tissue using anti-MEF2A antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse colon tissue using anti-MEF2A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/MEF2A-Antibody-(JA33-04)-Immunohistochemistry-Paraffin-NBP2-66943-img0004.jpg "Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse colon tissue using anti-MEF2A antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-MEF2A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/MEF2A-Antibody-(JA33-04)-Immunohistochemistry-Paraffin-NBP2-66943-img0005.jpg "Immunohistochemistry-Paraffin: MEF2A Antibody (JA33-04) [NBP2-66943] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-MEF2A antibody. Counter stained with hematoxylin.")
![Western Blot: MEF2C Antibody (1H5) [NBP2-00493] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEF2C.](http://images.novusbio.com/images/MEF2C Antibody (1H5)-Western Blot-NBP2-00493-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MEF2C Antibody (OTI1H5) [NBP2-00493] - MEF2C Antibody (1H5) [NBP2-00493] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEF2C.](http://images.novusbio.com/images2/MEF2C-Antibody-(1H5)-Immunocytochemistry-Immunofluorescence-NBP2-00493-img0010.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Human Ovary tissue using anti-MEF2C mouse monoclonal antibody.](http://images.novusbio.com/images/MEF2C Antibody (1H5)-Immunohistochemistry-Paraffin-NBP2-00493-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Human myoblasts were stained with MyoD1 antibody, diluted 1:200. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/MyoD-Antibody-(5.8A)-Immunocytochemistry-Immunofluorescence-NB100-56511-img0008.jpg)
![Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Insuline-like growth factor (IGF1) antagonized the effects of miR-106a-5p on myogenic differentiation in C2C12. All data were collected from C2C12 myotubes 5 d post differentiation. Western-blot analysis of myogenic regulatory factors (MyoD, MyoG, MyHC) in cells; (H) The statistical results of Figure 3G. Data were presented as mean +/- SEM. n = 3 per group. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4425/9/7/333/htm) licensed under a CC-BY licence. anti-MyoG (catalog# NB100-56510)](http://images.novusbio.com/images2/MyoD-Antibody-(5.8A)-Western-Blot-NB100-56511-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Cell Lines Tested: mouse skeletal muscle-derived primary cell population Test Sample Preparation: mouse skeletal muscle digested by collagenase type 2 System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan) Excitation Wavelength: 488nm Emission Filter: 562 nm. Image using the DyLight 488 form of this antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/MyoD-Antibody-(5.8A)-Immunocytochemistry-Immunofluorescence-NB100-56511-img0007.jpg)
![Western Blot: MEF2D Antibody [NBP1-85788] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/images2/MEF2D-Antibody-Western-Blot-NBP1-85788-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MEF2D Antibody [NBP1-85788] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/MEF2D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85788-img0008.jpg)
![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85788] - Staining of human kidney shows strong nuclear postivity in cells in glomeruli.](http://images.novusbio.com/images2/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85788-img0025.jpg)
![Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line (Rh30). Negative cell lines were PFSK-1, EB2, SK-N-SH, and SJSA-1.](http://images.novusbio.com/images2/Myogenin-Antibody-(5FD)-Western-Blot-NB100-56510-img0010.jpg)
![Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Human myocytes using NB100-56703 at 2.5 ug/mL. Hematoxylin-eosin counterstain.](http://images.novusbio.com/images2/Myogenin-Antibody-(5FD)-Immunohistochemistry-NB100-56510-img0009.jpg)
![Immunohistochemistry-Frozen: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin in human alveolar rhabdomyosarcoma using NB100-56510 at 5 ug/mL.](http://images.novusbio.com/images2/Myogenin-Antibody-(5FD)-Immunohistochemistry-NB100-56510-img0008.jpg)
![Western Blot: Serum Response Factor SRF Antibody [NBP1-71927] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SRF antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SRF was also immunoprecipitated by rabbit anti-SRF antibody NBP1-71928, which recognizes a downstream epitope.](http://images.novusbio.com/images2/Serum-Response-Factor-SRF-Antibody-Western-Blot-NBP1-71927-img0001.jpg)
![Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-71927] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SRF used at a dilution of 1:1,000 (1ug/ml) (left) and used at a dilution of 1:1,000 (1ug/ml) (right). Detection: DAB](http://images.novusbio.com/images2/Serum-Response-Factor-SRF-Antibody-Immunohistochemistry-Paraffin-NBP1-71927-img0002.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.](http://images.novusbio.com/images2/Glut4-Antibody-Western-Blot-NBP1-49533-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Glut4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49533-img0011.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney](http://images.novusbio.com/images2/Glut4-Antibody-Immunohistochemistry-NBP1-49533-img0005.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Analysis of HDAC4 in NIH/3T3 lysate.](http://images.novusbio.com/images2/HDAC4-Antibody-Western-Blot-NBP2-22151-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against DyLight 488 (Green) using a 40X objective. Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/images2/Histone-Deacetylase-4-HDAC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22151-img0005.jpg)
![Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Analysis of HDAC4 in mouse pancreas.](http://images.novusbio.com/images2/HDAC4-Antibody-Immunohistochemistry-NBP2-22151-img0002.jpg)
![Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.](http://images.novusbio.com/images2/HDAC5-Antibody-Western-Blot-NBP2-22152-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/images2/HDAC5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22152-img0001.jpg)
![Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.](http://images.novusbio.com/images2/HDAC5-Antibody-Immunohistochemistry-NBP2-22152-img0002.jpg)
![Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/images2/HDAC9-Antibody-Western-Blot-NBP2-03993-img0004.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Western-Blot-NBP2-37576-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Immunohistochemistry-Paraffin: MEF2B Antibody [NBP1-93440] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/images2/MEF2B-Antibody-Immunohistochemistry-Paraffin-NBP1-93440-img0006.jpg)
![Immunohistochemistry-Paraffin: MEF2B Antibody [NBP1-93440] - Staining of human lymph node shows strong nuclear cytoplasmic in reaction center cells.](http://images.novusbio.com/images2/MEF2B-Antibody-Immunohistochemistry-Paraffin-NBP1-93440-img0002.jpg)
![Immunohistochemistry-Paraffin: MEF2B Antibody [NBP1-93440] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/images2/MEF2B-Antibody-Immunohistochemistry-Paraffin-NBP1-93440-img0003.jpg)
![Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Hela cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the -39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.](http://images.novusbio.com/images2/p38-[p-Thr180--p-Tyr182]-Antibody-Western-Blot-NB500-138-img0005.jpg)
![Immunohistochemistry-Paraffin: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red.](http://images.novusbio.com/images2/p38 [p Thr180- p Tyr182] Antibody-Immunohistochemistry-Paraffin-NB500-138-img0002.jpg)
![Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182.](http://images.novusbio.com/images2/p38 [p Thr180- p Tyr182] Antibody-Western Blot-NB500-138-img0001.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Western Blot: MYF6 Antibody [NBP1-52030] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MYF6 with MYC tag probed with EB09957 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB09957 (0.5mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/MYF6-Antibody-Western-Blot-NBP1-52030-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Western Blot: SLC2A4RG Antibody (4C10) [H00056731-M02] - SLC2A4RG monoclonal antibody (M02), clone 4C10 Analysis of SLC2A4RG expression in HeLa.](http://images.novusbio.com/images2/SLC2A4RG Antibody (4C10)-Western Blot-H00056731-M02-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SLC2A4RG Antibody (4C10) [H00056731-M02] - Analysis of monoclonal antibody to SLC2A4RG on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/SLC2A4RG-Antibody-(4C10)-Immunocytochemistry-Immunofluorescence-H00056731-M02-img0004.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")