MED4 Antibody (2B10) - Azide and BSA Free

Western Blot: MED4 Antibody (2B10) [H00029079-M01] - Analysis of MED4 expression in transfected 293T cell line by MED4 monoclonal antibody (M01), clone 2B10.Lane 1: MED4 transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MED4 Antibody (2B10) [H00029079-M01] - Analysis of monoclonal antibody to MED4 on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: MED4 Antibody (2B10) [H00029079-M01] - Detection limit for recombinant GST tagged MED4 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MED4 Antibody (2B10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MED4 Antibody (2B10) - Azide and BSA Free (H00029079-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MED4 (AAH05189, 1 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAASSSGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDGDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKEDEDDVEIMSTDSSSSSSESD
• Specificity: MED4 - mediator of RNA polymerase II transcription, subunit 4 homolog (yeast) (2B10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MED4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED4 Antibody (2B10) - Azide and BSA Free

• Activator-recruited cofactor 36 kDa component
• ARC36
• DRIP36
• DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae)
• FLJ10956
• HSPC126
• HSPC126vitamin D receptor interacting protein
• MED4
• mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit
• subunit 4 homolog
• TRAP36
• VDRIP

Background

MED4 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED4
• Entrez:
  • 29079(Human)
• Uniprot:
  • AAH05189(Human)