MED24 Antibody

Product Discontinued

MED24 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 801-850 of Human TRAP100.
• Specificity: MED24 antibody detects endogenous levels of MED24 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED24
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  MED24 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MED24 Antibody

• cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa
• CRSP100
• DRIP100CRSP complex subunit 4
• hTRAP100
• KIAA0130CRSP4
• mediator complex subunit 24ARC100
• MGC8748
• subunit 24 homolog
• thyroid hormone receptor associated protein 4
• Thyroid hormone receptor-associated protein 4
• Thyroid hormone receptor-associated protein complex 100 kDa component
• Trap100
• TRAP100THRAP4
• Vitamin D3 receptor-interacting protein complex 100 kDa component
• vitamin D3 receptor-interacting protein complex component DRIP100

Background

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size from 20 to 240 kDa, including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, and they are characterized by the presence of a nuclear receptor recognition motif, which mediates the ligand-dependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR-induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MED24 Antibody (NB100-92293)(1)

Publication using NB100-92293

• El Khattabi L, Zhao H, Kalchschmidt J et al. A Pliable Mediator Acts as a Functional Rather Than an Architectural Bridge between Promoters and Enhancers Cell 2019-08-22 [PMID: 31402173]

Bioinformatics

• Gene Symbol: MED24
• Entrez:
  • 9862(Human)
  • 23989(Mouse)
  • 619436(Rat)
• Uniprot:
  • O75448(Human)