Recombinant Human MED21 Protein

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SDS-Page: MED21 Protein [NBP2-23221]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human MED21 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-144 of Human MED21

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
MED21
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human MED21 Protein

  • hSrb7
  • mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog
  • RNA polymerase II complex component SRB7
  • RNA polymerase II holoenzyme component SRB7
  • RNAPII complex component SRB7
  • SRB7 suppressor of RNA polymerase B homolog
  • SRB7SRB7 suppressor of RNA polymerase B homolog (yeast)
  • SURB7mediator of RNA polymerase II transcription subunit 21

Background

MED21 is a member of the mediator complex subunit 21 family. The protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. Recombinant human MED21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics Tool for MED21 Protein (NBP2-23221)

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Bioinformatics

Gene Symbol MED21