Recombinant Human MED21 His Protein

SDS-Page: Recombinant Human MED21 His Protein [NBP2-23221] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human MED21 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-144 of Human MED21
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MED21
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MED21 His Protein

• hSrb7
• mediator complex subunit 21SRB7 (suppressor of RNA polymerase B, yeast) homolog
• RNA polymerase II complex component SRB7
• RNA polymerase II holoenzyme component SRB7
• RNAPII complex component SRB7
• SRB7 suppressor of RNA polymerase B homolog
• SRB7SRB7 suppressor of RNA polymerase B homolog (yeast)
• SURB7mediator of RNA polymerase II transcription subunit 21

Background

MED21 is a member of the mediator complex subunit 21 family. The protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. Recombinant human MED21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MED21